摘要
为了分析研究白介素25(IL-25)、白介素33(IL-33)和嗜酸性细胞趋化因子(Eotaxin)在哮喘儿童发病中起到的作用,本研究选取了我院收治的哮喘儿童患者90例,分为急性发作期30例,健康对照组30例,临床缓解期30例。用双抗体夹心ELISA法检测三组哮喘儿童患者的血清里面IL-25、IL-33、Eotaxin的表达情况,同时对三者在患儿血清水平中的关联性加以分析。在急性发作期组的30例哮喘儿童患者血清中IL-25、IL-33的水平明显高于正常对照组的30例和临床缓解组的30例,差异具有统计学意义,临床缓解组与正常对照组相比较差异没有统计学意义;在急性发作期哮喘儿童患者血清中Eotaxin的水平与临床缓解组和正常对照组相比较明显要高,有统计学意义,临床缓解组和正常对照组相比较,没有统计学意义。哮喘患儿的血清中IL-25、IL-33的水平与嗜酸性细胞趋化因子(Eotaxin)的水平属于正相关的关系。白介素25(IL-25)、白介素33(IL-33)和嗜酸性细胞趋化因子(Eotaxin)在儿童哮喘发病过程中发挥着重要的作用。三者在患儿血清中的表达水平与哮喘气道炎性反应密切关联,可以考虑作为潜在的靶点应用在儿童哮喘的临床治疗中。
In order to investigate the role ofinterleukin -25 （IL-25）, interleukin-33 （IL-33） and eotaxin in the pa- thogenesis of asthma in children with asthma, this study selected 90 children with asthma cases were divided into acute exacerbation of 30 cases, 30 cases of healthy control group, 30 cases of clinical remission.The levels of IL-25, IL-33 and Eotaxin in the serum of the asthmatic children were detected by double antibody sandwich ELISA, and the correlation between the serum levels oflL-25, IL-33 and Eotaxin was also analyzed. The levels oflL-25 and IL-33 in serum of 30 asthmatic children in acute attack group were significantly higher than those in normal control group and 30 cases of clinical remission group, the difference was statistically significant, clinical remission group and normal. There was no statistically significant difference between the control group and the control group; The serum levels of Eotaxin in asthmatic children were significantly higher than those in clinical remission group and normal control group. There was no statistical significance between clinical remission group and normal control group. Levels of IL-25 and IL-33 in serum of asthmatic children were positively correlated with the levels of eotaxin （Eotaxin）. Interleukin-25 （IL-25）, interleukin-33 （IL-33） and eotaxin （Eotaxin） play an important role in the pathogenesis of childhood asthma. The expression level of the three proteins in children with asthma is closelyrelated to the airway inflammation of asthma, so it can be considered as a potential target in the clinical treatment of childhood asthma.
作者
倪凯华
Ni Kaihua(Affiliated Hospital of Nantong University, Nantong, 226001)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第11期4464-4468,共5页
Genomics and Applied Biology
基金
江苏南通大学附属医院资助
