摘要
目的:研究过表达集落刺激因子1受体(colony stimulating factor-1 receptor,CSF-1R)对人鼻咽癌(nasopharyngeal carcinoma,NPC)6-10B细胞凋亡的抑制作用及其与Bax和Bcl-2表达之间的关系。方法:体外利用慢病毒构建的CSF-1R过表达载体LV-CSF1R(16957-1)转染到人鼻咽癌6-10B细胞中,实验分转染组和对照组;采用实时荧光定量PCR及Western blotting检测转染后两组细胞中CSF-1R、Bcl-2、Bax的表达情况;CCK-8法检测两组细胞的增殖;流式细胞术检测两组细胞的凋亡情况。结果:转染组的6-10B细胞中其CSF-1 mRNA表达水平明显高于对照组(7.01±0.23 vs 0.09±0.03,P<0.01);Bax mRNA表达水平显著下调(P<0.01),而Bcl-2 mRNA表达水平显著上调(P<0.01)。转染组的6-10B细胞中CSF-1蛋白表达水平明显高于对照组;Bax蛋白表达水平显著下调,而Bcl-2蛋白表达水平稍上调。与对照组相比,转染组6-10B细胞的增殖活力明显提高(P<0.01);凋亡率显著降低[(10.82±0.75)%vs(17.11±0.46)%,P<0.05]。结论:过表达CSF-1R可以通过调节Bax/Bcl-2之间的比例关系来促进鼻咽癌6-10B细胞的恶性增殖,并抑制细胞凋亡。
Objective： To investigate the inhibitory effect of Colony stimulating factor- 1 receptor （CSF- 1R） on apoptosis of human nasopharyngeal carcinoma 6-10B cells and its relationship with Bax/Bcl-2 expression. Methods：Lentiviral vector over- expressing CSF-1R LV-CSF1R（16957-1）was constructed and trasfected into 6-10B cells; meanwhile a control group was set; The expression of CSF-1R, Bcl-2 and Bax was detected by Real-time PCR and Western blotting. CCK-8 assay and flow cytometry （FCM） were used to detect the cell proliferation and apoptosis,respectively. Results： The mRNA expression of CSF-1R increased significantly in 6-10B cells of transfection group compared with negative control （NC） group （7.01±0.23 vs 0.09±0.03, P〈0.01）; The expression of Bax mRNA was significantly down-regulated while the expression of Bcl-2 mRNA was significantly up-regulated in 6-10B cells of transfection group （all P〈0.01）. The protein expression of CSF-1 in 6-10B cells of transfection group was significantly higher than that of control group, along with the remarkably down- regulated Bax protein expression and slightly up-regulated Bcl-2 protein in transfection group. Compared with NC group, the proliferation vitality of 6-10B cells were extremely increased after transfection （P〈0.01）; however, the cell apoptosis was significantly reduced （[10.82±0.75]% vs [17.11±0.46]%, P〈0.05）. Conclusion： Over-expression of CSF-1R could promote the ma-lignant growth and inhibit the apoptosis of nasopharyngeal carcinoma 6-10B cells by regulating the ratio of Bax/Bcl-2 expression.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2017年第12期1386-1390,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No.81260348)~~
