摘要
以黑龙江扎龙湿地土壤为试材,采用形态特征观察、生理生化特性研究和16S rDNA序列系统发育分析的方法,研究了培养温度重金属、浓度、NaCl浓度、pH、抗生素种类和氧气对菌株生长的影响,以期为利用耐铅、镉菌株治理重金属污染提供参考依据。结果表明:筛选出的耐金属菌株初步鉴定其为Burkholderiasp.,菌株最适温度为30℃,最适pH为7,可以在含盐量<6%的培养基上生长。随着NaCl浓度的升高,该菌株生长受到抑制。菌株可能属于好氧菌。对于其它重金属,在低浓度含Cu^(2+)培养基生长,高浓度抑制,对Cr^(6+)不具耐性。该菌株能在含有不同低浓度的抗生素环境下生长,高浓度时抑制生长。
This experiment is based on the soil of Zalong wetland in Heilongjiang Province,using the method of state characteristic observation,physiological and biochemical characteristics and the development analysis of 16 S rDNA sequence systems,the effects of temperature,heavy metal concentration,NaCl concentration,pH,antibiotic type and oxygen on the growth of strain were studied,in order to provide reference for the use of lead-resistant and cadmium strains to control heavy metal pollution.The results showed that a strain resistant to heavy metals was screened and initially identified as Burkholderiasp,the optimum temperature was 30℃and the optimum pH was 7.It could be grown on medium with salt content〈6%.With the increase of NaCl concentration,the growth of the strain was inhibited.The strain might belong to aerobic bacteria.For other heavy metals,in the low concentration of Cu^2＋ containing medium growth,high concentration inhibition,not resistant to Cr^6＋.The strain could grow in an environment containing different concentrations of antibiotics,high concentrations were inhibited when grew.
作者
金忠民
赵婧佟
温昱晨
聂文军
刘丽杰
JIN Zhongmin;ZHAO Jingtong;WEN Yuchen;NIE Wenj un;LIU Lijie(College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang 161006)
出处
《北方园艺》
CAS
北大核心
2017年第24期65-71,共7页
Northern Horticulture
