摘要
目的 细胞死亡标记物M65和M30已证实对非酒精性脂肪性肝病和慢性丙肝患者的肝脏纤维化具有一定诊断意义,而对慢性乙肝患者的研究较少,本研究旨在明确M65和M30在慢性乙型肝炎患者不同肝脏纤维化分组间的差异比较,以探索其潜在的诊断价值.方法 我们收集186例行肝脏穿刺检查的慢性乙型肝炎患者,肝脏炎症程度分为G1至G4级,肝脏纤维化程度分为S1至S4期,并在肝穿前后1周时间内采血,同时选择性别和年龄匹配的18例正常人作为健康对照,通过M65 EpiDeath ELISA和M30 Apoptosense ELISA试剂盒,对所有人群进行血清M65、M30等指标检测.结果 血清M65水平在S2组和cS3组的中位水平分别为246.7 U/L和226.5 U/L,均明显高于S1组（中位水平为199.6 U/L）和cS3组（中位水平为144.5 U/L）.结论 血清M65水平随纤维化程度加重而升高,可能对慢性乙型肝炎患者肝纤维化程度具有辅助诊断价值.
Objective The markers of cell death,M65 and M30,have been suggested to be sensitive markers of liver fibrosis in nonalcoholic fatty liver disease and chronic hepatitis C.Only few studies were carried out on chronic hepatitis B （CHB） and the results were inconsistent.Our aim was to investigate if these markers showed difference in different stages of fibrosis in CHB patients.Methods We examined 186 patients with CHB.Eighteen gender and age matched healthy subjects were used as controls.Blood samples were collected from CHB patients one week before or after liver biopsy.Liver inflammation was graded from G1 to G4,and fibrosis was staged from S1 to S4.The serum levels of M65 and M30 were quantitatively measured using the M65 EpiDeath（R） ELISA kit and the M30 Apoptosense（R） ELISA kit according to the manufacturer＆#39;s protocol.Results The median level of serum M65 in S2 and cS3 groups were 246.7U/L and 226.5U/L,respectively,and both were higher than that in S1 group （199.6U/L） and in control group （144.5U/L）.Conclusions Serum M65 increased with the degree of liver fibrosis and maybe a potential indicator of liver of fibrosis.
出处
《国际病毒学杂志》
2017年第6期361-364,共4页
International Journal of Virology
