摘要
以发芽糙米为原料,用超声波辅助复合酶的方法提取发芽糙米中的多糖类物质,以发芽糙米多糖得率为指标进行单因素试验,并通过响应曲面试验确定超声波辅助复合酶法提取发芽糙米多糖的最佳工艺条件,通过测定DPPH自由基、超氧阴离子和羟基自由基的清除能力,检测提取的多糖抗氧化活性。结果表明:复合酶(木瓜蛋白酶∶纤维素酶:果胶酶的添加质量比例为4∶3∶2)的酶解pH值为6,酶解时间2.5 h,酶解温度为50℃,酶添加量为1.5%,此条件下多糖得率为37.58%。抗氧化性研究发现发芽糙米多糖对DPPH自由基和超氧阴离子,羟基自由基都具有较强的抗氧化作用。
The germinated brown rice is the raw material,extract polysaccharides from brown rice germination by ultrasonic assisted method of compound enzyme,the germinated brown rice yield of polysaccharide was the index of single factor experiment,and through the response surface experiment to determine the optimum conditions.Meanwhile,the anti-oxidative activity of polysaccharides were detected. The results showed that the optimal ultrasonic-assisted extraction parameters were composite enzyme of(papaya protease,cellulase,pectinase adding quantity proportion for 4 ∶ 3 ∶ 2)at 1.5 % dosage for 2.5 h at pH 6 and 50 ℃. Under such conditions,the maximum yield of polysaccharides from the germinated brown rice was 37.58 %. The polysaccharide of germinated brown rice had strong clearance power to OH^-,O^(2-)and DPPH free radicals.
出处
《食品研究与开发》
CAS
北大核心
2018年第1期30-37,共8页
Food Research and Development
基金
哈尔滨商业大学校级科研项目(17XN023)
关键词
发芽糙米
多糖
超声波
复合酶法
响应曲面法
抗氧化性
germinated brown rice
polysaccharides
ultrasound
enzyme complex method
response surfacemethod
antioxidant activity
