期刊文献+

HPLC波长切换法同时测定金嗓散结胶囊中的(R,S)-告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A 被引量:4

Simultaneous Determination of( R,S)-goitrin,Hydroxysafflor Yellow A,Salvianolic acid B,Baicalein-7-O-diglucoside and Baicalin-7-glucoside in Jinsang Sanjie Jiaonang by HPLC Wavelength Switching Method
下载PDF
导出
摘要 目的:建立金嗓散结胶囊中(R,S)-告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A的同时测定方法。方法:采用HPLC法,应用Agilent HC-C18(250 mm×4.6 mm,5μm)色谱柱;以甲醇-乙腈(3∶1)与0.4%磷酸溶液为流动相,进行梯度洗脱;流速为1.0 m L·min^(-1);供试品采用50%甲醇超声提取。结果:(R,S)告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A的线性范围分别为4.150~83.00、5.120~102.4、11.07~221.4、4.590~91.80、8.140~162.8μg·m L^(-1),相关系数分别为0.999 2、0.999 9、0.999 4、0.999 7、0.999 5;5种成分分离良好,阴性对测定无干扰,平均加样回收率及相应的RSD分别为99.06%(1.27%)、99.54%(1.15%)、98.19%(0.89%)、97.88%(1.71%)、97.22%(0.99%)。结论:所建立的方法可用于金嗓散结胶囊的质量控制。 Objective:To develop a method for simultaneous determination of the content of (R,S)-goitrin,hydroxysafflor yellow A,salvianolic acid B,baicalein-7-O-diglucoside and baicalin-7-glucoside in Jinsang Sanjie Jiaonang.Methods:An HPLC method was adopted for the analysis,which was performed on a Agilent HC-C18(250 mm×4.6 mm,5.0 μm)chromatographic column with the mobile phase of methanol-acetonitrile(3∶1)(A)-0.4%phosphoric acid solution(B)in a gradient elution,the flow rate of 1.0 mL·min-1,and the samples were preprocessed with ultrasonic extraction in 50% methanol solution.Results:The linearity ranges of(R,S)-goitrin,hydroxysafflor yellow A,salvianolic acid B,baicalein-7-O-diglucoside and baicalin-7-glucoside were 4.150-83.00 μg·mL-1(r=0.999 2),5.120-102.4 μg·mL-1(r=0.999 9),11.07-221.4 μg·mL-1(r=0.999 4),4.590-91.80 μg·mL-1(r=0.999 7),8.140-162.8 μg·mL-1(r=0.999 5),respectively.The five components were separated perfectly without disturbing peaks from negative samples.The average recoveries and the corresponding RSD were 99.06%(1.27%),99.54%(1.15%),98.19%(0.89%),97.88%(1.71%),97.22%(0.99%),respectively.Conclusion:The method can be applied to the quality control of Jinsang Sanjie Jiaonang.
出处 《中国现代中药》 CAS 2017年第12期1758-1761,1770,共5页 Modern Chinese Medicine
基金 肇庆市科技创新指导类项目[肇科(2017)33号文]
关键词 金嗓散结胶囊 (R S)-告依春 羟基红花黄色素A 丹酚酸B 木蝴蝶苷B 木蝴蝶苷A 波长切 换法 中成药多组分测定 Jinsang Sanjie Jiaonang .(R, S)-goitrin hydroxysafflor yellow A salvianolic acid B baicalein-7-O-diglucoside baicalin-7-glucoside wavelength switching method multicomponent determination of Chinese patent drug
  • 相关文献

参考文献10

二级参考文献92

共引文献142

同被引文献46

引证文献4

二级引证文献17

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部