摘要
目的 评价带状疱疹减毒活疫苗的临床前安全性及免疫原性。方法 用不同剂量带状疱疹减毒活疫苗皮下接种BALB/c小鼠1或2次，观察接种后的临床症状，并在末次接种后2周采集并分离小鼠血清和脾淋巴细胞。采用荧光抗体膜抗原法和糖蛋白ELISA检测血清抗水痘-带状疱疹病毒抗体水平。用酶联免疫斑点试验检测分泌IFN-γ的T细胞数量。用流式细胞仪检测不同T细胞所占比例。结果 疫苗接种后未见不良反应发生，且小鼠的T细胞平衡未受到影响。无论接种1次还是2次，都能诱导体液和细胞免疫应答，且再次接种后诱导的免疫应答更强。随着免疫剂量的增加，疫苗诱导的免疫应答也随之增强。结论 带状疱疹减毒活疫苗具有较好的安全性和免疫原性。
Objective To evaluate the preclinical safety and immunogenicity of live attenuated herpes zoster （HZ） vaccine. Method BALB/c mice were immunized subcutaneously with different doses of herpes zoster vaccine 1 or 2 times. The clinical presentation was observed. The serum and spleen lymphocytes were collected at 2 weeks after last immunization. The antibody against varicella-zoster virus in serum was detected by fluorescent antibody membrane antigen method and glycoprotein ELISA. The numbers of IFN-γ-secreting T cells were detected by enzyme-linked immunospot assay. The frequency of different T cells was measured by flow cytometry. Results There were no side effects in BALB/c mice with 1 or 2 vaccinations. The balance of T cells was not affected in mice. Humoral and cellular immune responses in mice were induced by both 1 and 2 immunizations, and enhanced by booster injection. The dose of vaccine was correlated with the strength of humoral and cellular immune responses. Conclusion The live attenuated herpes zoster vaccine has good safety and immunogenicity.
出处
《国际生物制品学杂志》
CAS
2017年第6期261-264,共4页
International Journal of Biologicals
基金
上海市科技支撑项目（16431904500）
关键词
带状疱疹疫苗
安全
免疫
体液
免疫
细胞
Herpes zoster vaccine
Safety
Immunity, humoral
Immunity, cellular
