摘要
利用毛细管电泳(CE)技术建立了全氟辛酸(PFOA,C_8HF_(15)O_2)与人血清白蛋白(HSA)相互作用的分析方法。在生理条件下构建配体(PFOA)-受体(HSA)相互作用模型,通过淌度移动法、区段-区段动力学(plug-plug kinetic,PPK)法、简化的Hummel-Dreyer(HD)法研究其与HSA的相互作用。简化的HD法运用非线性方程、Scatchard方程和Klotz方程获得PFOA-HSA体系的相互作用参数,进而分析了模型适用度。结果表明,淌度移动法、PPK法、简化的HD法均适用于PFOA-HSA体系相互作用的分析,其中简化的HD法最优。模型适用度分析得出非线性回归方程为最适理论模型。相互作用参数测试表明PFOA-HSA相互作用体系之间发生的结合反应只有单一类型的结合位点且结合稳定。相关工作阐明了人血清白蛋白与PFOA的相互作用机制,可为PFOA毒理机制的深入研究提供有益参考。
Capillary electrophoresis(CE)has been used to establish the analytical method of interaction between pentadecafluorooctanoic acid(PFOA)and human serum albumin(HSA).Under the physiological conditions,the interaction model of PFOA and HSA were constructed.Mobility method,plug-plug kinetic(PPK)method and simplified Hummel-Dreyer method were used to determine the interaction between derivatives and HSA. Non-linear regression,Scatchard equation and Klotz equation were adopted to obtain the interaction parameters. The results showed that all the three methods can be used to analyze the interaction of PFOA-HSA system. According to the interaction parameters,the most suitable CE method is simplified Hummel-Dreyer method while the most suitable theoretical equation is non-linear regression.The binding parameters indicated that the interaction of PFOA-HSA system has only one type of binding sites and the binding is stable. The research results have illustrated the interaction between HSA and PFOA,and provided a beneficial reference for in-depth research of the toxic mechanism of PFOA.
出处
《色谱》
CAS
CSCD
北大核心
2018年第1期69-77,共9页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(20877072)~~
关键词
毛细管电泳
全氟辛酸
人血清白蛋白
结合参数
capillary electrophoresis(CE)
pentadecafluorooctanoic acid(PFOA)
human serum albumin(HSA)
binding parameters
