摘要
本文利用三种不同极性溶剂从石上柏中分离出活性成分,观察其对喉癌Hep-2细胞的增殖与迁移作用的影响。干燥的石上柏全草粉碎后经乙醇回流提取浓缩得浸膏,依次采用石油醚、二氯甲烷、乙酸乙酯三种溶剂进行萃取,以获得各极性溶剂萃取部位;MTT法检测石上柏提取物对Hep-2细胞增殖的影响;流式细胞仪检测石上柏提取物对Hep-2细胞周期的影响;细胞划痕实验检测石上柏提取物对Hep-2细胞迁移能力的影响;采用p53报告基因探讨石上柏提取物对喉癌Hep-2细胞的作用机制。结果显示31.2~500.0μg/m L的乙酸乙酯萃取部位能明显抑制Hep-2细胞的生长及划痕区域的迁移;15.0~60.0μg/mL的乙酸乙酯萃取物部位能使喉癌Hep-2细胞阻滞于G1期,并且能激活p53的表达。结果表明石上柏乙酸乙酯萃取部位可明显抑制喉癌Hep-2细胞的生长及迁移,其作用与激活p53通路有关。
In this study,SelagineUa doederleinii was extracted by three reagents with different hydrophobicity. The effects of these extracts on proliferation, cell cycle and migration of 14ep-2 cells in vitro were assessed. The petroleuu; ether, di- chloromethane and ethyl acetate fractions were prepared from ethanol extract of S. doederleinii. The effect of these fractions on proliferation of Hep-2 cells was determined by MTT assay;cell cycle was measured by flow cytometry （FCM） ; the anti-migration ability was observed through cell scratch method;and the molecular mechanism was determined by p53 reporter gene. The results showed that the ethyl acetate extract （31.2-500.0μg/mL） showed the best anti-tumor activi- ty in vitro and it can induce the inhibition of cell migration. Ethyl acetate extract （ 15.0-60.0μg/mL） also can induce cell cycle attest and activate the expression of io53. It indicated that the ethyl acetate extract of S. doederleinii can signifi- cantly inhibit tile growth and migration of I-lep-2 cells through a p53 pathway.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第12期2024-2029,共6页
Natural Product Research and Development
基金
湖南省大学生研究性学习和创新性实验计划(2017-205)
湖南医药学院青年优秀科研人才支持计划(2013-85)
关键词
石上柏
喉癌
细胞增殖
细胞迁移
细胞周期
Selaginella doederleinii
laryngocarcinoma
proliferation
migration
cell cycle
