摘要
利用蛋白酶对脱脂柞蚕蛹蛋白进行酶解制备具有活性的多肽,可以提高柞蚕蛹蛋白的应用价值。为了建立适合工业化应用的脱脂柞蚕蛹蛋白的酶解工艺条件,首先采用单因素试验考察料液质量浓度、复合中性蛋白酶种类与使用浓度、酶解温度和时间4个因素对脱脂柞蚕蛹蛋白水解度的影响,得出较适合的工艺条件是:料液质量浓度60 g/L,3 g/L复合中性蛋白酶中的风味蛋白酶与蚕蛹专用复配蛋白酶按1∶1质量比混合,酶解温度55℃,酶解时间10 h。进一步采用Box-Behnken设计及响应面分析法对工艺条件进行优化,在复合中性蛋白酶质量浓度4.85 g/L、料液质量浓度41 g/L、酶解温度55℃、酶解时间10.66 h的最佳酶解条件下,脱脂柞蚕蛹蛋白的理论水解度为45.82%,实际水解度为45.75%。利用优化的酶解工艺条件制备柞蚕蛹蛋白活性肽,具有酶解效率高、稳定性好、操作简单、生产成本低的特点。
The application value of tussah pupa proteins can be improved by using protease to hydrolyze skim tussah pupa proteins for preparation of active polypeptides. In order to establish process conditions suitable for industrial enzymolysis of skim tussah pupa proteins,single factor experiments were conducted to investigate the effects of mass concentration of feed solution,compound neutral protease type and concentration,hydrolysis temperature and hydrolysis time on hydrolysis degree of skim tussah pupa proteins. The optimal hydrolysis conditions were as follows： 60 g/L mass concentration of feed solution,3 g/L of flavourzyme in compound neutral protease mixed with silkworm-pupa-specific compound protease at 1 ∶ 1 mass ratio,hydrolysis temperature 55 ℃ and hydrolysis time 10 h. Box-Behnken design and response surface methodology were further employed to optimize the hydrolysis conditions. It was found that,under 4. 85 g/L compound neutral protease concentration,41 g/L mass concentration of feed solution, 55 ℃ hydrolysis temperature and 10. 66 h hydrolysis time,the theoretical hydrolysis degree of skim tussah pupa proteins was45. 82%,and the actual hydrolysis degree was 45. 75%.Preparation of active peptides from hydrolyzing skim tussah pupa proteins under optimized process condition has the characteristics of high efficiency,good stability,simple operation and low production cost.
出处
《蚕业科学》
CAS
CSCD
北大核心
2017年第6期991-997,共7页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系建设专项(No.CARS-18)
辽宁省重大科技攻关项目(No.2014209001)
关键词
脱脂柞蚕蛹蛋白
多肽
复合中性蛋白酶
酶解工艺
响应面分析法
水解度
Skim tussah pupa protein
Polypeptide
Compound neutral protease
Enzymolysis technology
Responsesurface methodology
Hydrolysis degree
