摘要
利用磷酸水解的方法将柞蚕丝分别水解4 h和6 h后,得到不同分子质量的1号柞蚕丝肽和2号柞蚕丝肽。将2种柞蚕丝肽分别按照低、中、高3个剂量[1.0、2.5、5.0 g/(kg·d)]给小鼠连续灌胃给药30 d,测试各试验组小鼠负重游泳时间及小鼠血清中尿素氮与肝糖原的含量,评价柞蚕丝肽抗疲劳的功效。与给予等体积蒸馏水的对照组(CK)相比,除2号柞蚕丝肽中剂量[2.5 g/(kg·d)]试验组外,其他试验组小鼠的体质量均显著下降(P<0.05);1号柞蚕丝肽3个剂量试验组小鼠的负重游泳时间均极显著延长(P<0.01),2号柞蚕丝肽3个剂量试验组小鼠的负重游泳时间也呈现延长趋势,但差异不显著(P>0.05);1号柞蚕丝肽和2号柞蚕丝肽都能降低小鼠运动后血清尿素氮水平,其中低剂量组的效果要好于高剂量组,且1号柞蚕丝肽低剂量试验组的效果极显著(P<0.01);1号柞蚕丝肽、2号柞蚕丝肽各剂量试验组小鼠的肝糖原含量均有所提高,但差异不显著(P>0.05)。制备的2种柞蚕丝肽均有增强小鼠抗疲劳能力的趋势,且分子质量较大的1号柞蚕丝肽优于分子质量较小的2号柞蚕丝肽。
Tussah silk peptides were prepared from tussah（ Antheraea pernyi） silk through hydrolysis by phosphoric acid. Sample 1 and sample 2 with different molecular weight were obtained based on 4 h and 6 h of hydrolysis,respectively. Test mice were orally administered with low-,medium-and high-dose [1. 0,2. 5 and 5. 0 g/（ kg·d） ] tussah silk peptides of the two samples respectively for 30 d. Consequently,weight-loaded swimming time,index of serum urea nitrogen and liver glycogen of the test mice were detected to evaluate the anti-fatigue effect of tussah silk peptides. Compared with the control group which was supplied with equal volume of distilled water,the weight gain of mice was significantly decreased（ P0. 05） except the medium-dose group [2. 5 g/（ kg·d） ] of sample 2. The three dose groups of sample 1 were found to prolong the weight-loaded swimming time of mice significantly（ P0. 01）. The three dose groups of sample 2 also had a tendency of prolonging the weight-loaded swimming time of mice,but had no statistical significance（ P0. 05）. Both samples 1 and 2 could reduce the level of serum urea nitrogen in mice after exercise,among which the low-dose treatment was more effective than high-dose treatment and the effect of low-dose treatment of sample 1 reached significant level（ P 0. 01）.Treatment with both samples 1 and 2 could improve the content of hepatic glycogen of mice,though without statistical significance（ P0. 05）. Therefore,tussah silk peptides of both samples are able to improve the anti-fatigue function in mice. Moreover,tussah silk peptides with high molecular weight（ sample 1） exhibited better effect than those with low molecular weight（ sample 2）.
出处
《蚕业科学》
CAS
CSCD
北大核心
2017年第6期998-1003,共6页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系建设专项(No.CARS-18)
辽宁省重大科技攻关项目(No.2014209001)
关键词
柞蚕丝肽
磷酸水解
抗疲劳
运动时间
血清尿素氮
肝糖原
小鼠
Tussah silk peptide
Hydrolysis by phosphoric acid
Anti-fatigue
Exercising time
Serum urea nitrogen
Hepatic glycogen
Mouse
