摘要
目的采用兔耳缘静脉注射。肾上腺素的方法建立中心性浆液性脉络膜视网膜病变（CSC）动物模型，为CSC发病机制和防治研究提供动物模型。方法分别选取雄性新西兰白兔10只、比利时兔5只和青紫蓝兔10只，采用耳缘静脉注射荧光素钠法行荧光素眼底血管造影（FFA），排除眼底血管病变。新西兰白兔每天经耳缘静脉注射肾上腺素0．04mg／kg，连续注射8周，注射后9—12周肾上腺素每日注射剂量增至0．08mg／kg；比利时兔和青紫蓝兔均每天经耳缘静脉注射肾上腺素0．04mg／kg。实验兔每周行FFA，分别于注射后4、8和12周取新西兰白兔各3、3和4只眼球制作视网膜石蜡切片，分别于注射后8周取比利时兔5只眼球和青紫蓝兔10只眼球制备视网膜石蜡切片并行苏木精-伊红染色，观察各品系实验兔视网膜组织结构。结果新西兰白兔静脉注射肾上腺素后1～12周眼底均未见明显荧光素渗漏，组织病理学检查均未发现视网膜脱离。5只比利时兔中2只眼分别于注射后1周和2周出现眼底荧光素渗漏，组织病理学检查可见渗漏处视网膜神经上皮层脱离和视网膜色素上皮（RPE）脱色素，细胞间隙增大。10只青紫蓝兔在注射后8周内共有7只眼出现眼底荧光素渗漏、RPE脱色素、局部视网膜神经上皮层脱离。结论静脉注射肾上腺素在有色兔可诱导出CSC样病变，而在白化兔上无法诱导出CSC样病变。
Objective To establish a central serous ehorioretinopathy （CSC） model on different strains of rabbits by intravenous injection of adrenaline,which may contribute to related researches of CSC. Methods This study was approved by Bioethics Committee of Fourth Military University and complied with Statement for the Use of Animals in Ophthalmic and Visual Research; Fundus fluorescein angiography （FFA） was initially performed on male New Zealand white rabbits （10）, Belgium rabbits （5） and Chinchilla rabbits（10） to make sure that the retinas of subjects were normal. For the New Zealand white rabbits, adrenaline was injected via ear vein at a dose of 0. 04 mg/kg once per day for the first 8 weeks and followed by a dose of O. 08 mg/kg for the next 4 weeks, while 0. 04 mg/kg adrenaline was injected in the same way for 8 weeks in the Belgium rabbits and Chinchilla rabbits. FFA was performed every week after injection of adrenaline to evaluate the fluorescence leakage in ocular fundus. New Zealand white rabbits were sacrificed in 4 （3 rabbits）, 8 （3 rabbits） and 12 weeks （4 rabbits） after injection respectively, and Belgium rabbits and Chinchilla rabbits were sacrificed in the 8 weeks after injection. The eyeballs of the rabbits were enucleated to prepare the retinal sections for histopathological examination after hematoxylin-eosin staining. The results of FFA and retinal structure were compared among different strains of rabbits. Results No fluorescence leakage was found by FFA in ocular fundus, and the retinal structure was normal in all the 10 New Zealand white rabbits during the experiment. Fluorescence leakage was found by FFA in 2 Belgium rabbits at 1 week and 2 weeks after injection respectively, and retinal detachment and depigmentation of retinal pigment epithelium （RPE） with an enlarged intercellular space were shown by hematoxylin-eosin staining. For the Chinchilla rabbits, fluorescence leakages were found in 7 rabbits throughout the whole period of adrenaline administration. Circumscribed retinal detachment, depigmentation of RPE with enlarged intercellular space were also found in leakage lesions. Conclusions Repeated intravenous injection of adrenaline can induce CSC-like lesions in colored rabbits but not in albino rabbits.
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2018年第1期23-27,共5页
Chinese Journal Of Experimental Ophthalmology
基金
陕西省科技资源开放共享平台项目（2015FWPT-02）
