摘要
Ca^(2+)是植物细胞信号转导中重要的第二信使,在植株受到外界逆境胁迫时,细胞中的钙信号经钙传感蛋白(CaMs、CaMLs、CBLs和CDPKs)传递到下游组分引起相关基因的表达,从而响应环境的各种变化。钙依赖性蛋白激酶(CDPK)是Ca^(2+)传感器,可以将细胞内Ca^(2+)信号传递到可以与14-3-3蛋白相互作用的靶蛋白从而刺激靶蛋白发生磷酸化。CDPK在植物生长的诸多方面发挥关键作用,如花粉管的伸长、植物的生长发育以及对生物胁迫、非生物胁迫的应激反应。大多数CDPK具有明显的亚细胞分布,使它们能够"感受"局部Ca^(2+)浓度的变化并与其靶细胞发生特异性作用。14-3-3蛋白是一类通过磷酸化激活的真核蛋白,它可以与不同靶蛋白相互作用,调控植物重要的生理生化过程。近年来研究发现,14-3-3蛋白作为CDPK的靶蛋白和CDPK交叉磷酸化形成CDPK/14-3-3复合物,进一步调节植物初级代谢、开花和激素合成。将从CDPK的结构、亚细胞定位、靶蛋白、生物学功能,特别是CDPK与14-3-3之间的交叉调节及其在植物信号通路中的协同作用方面进行全面的综述,旨在为未来CDPK研究提供参考和新的方向。
Ca^(2+)is an important secondary messenger in signal transduction in plant cells.When plants are exposed to fluctuating environmental conditions Ca^(2+)signals are perceived and decoded by Ca^(2+)sensors(CaMs,CaMLs,CBLs and CDPKs)to elicit the expression level of related genes.Calcium-dependent protein kinase(CDPK)is a Ca^(2+)sensor playing apivotal role in plant development,pollen tube elongation and responses to abiotic and biotic stimuli.It has the unique ability to directly transmit cytolic Ca^(2+)signals to downstream phosphorylation events in diverse substrates which can mediate interaction with 14-3-3 proteins to modulate protein functions.Most CDPKs have significant subcellular distribution,allowing them to"feel"local Ca^(2+)concentration and to act specifically with their target cells.14-3-3 proteins are highly conserved in eukaryotic cells,which in most cases need to bind to different targets and be phosphorylated to modulate their activity.Through protein-protein interactions,14-3-3 proteins are involved in many significant physiological processes in plants.Recent studies have revealed that the role of CDPK in phosphorylating sites in mediating 14-3-3 protein binding to form CDPK/14-3-3 complex,and have also highlighted the role of the CDPK/14-3-3 complex in regulating primary metabolism,plant hormone synthesis and flowering.In this paper,CDPK structure,subcellular localization,target protein,biological functions,especially the cross regulation between CDPK and 14-3-3 and their synergistic effect in plant signaling pathway will be discussed in depth.Our study aims are to provide a reference and indicate new directions for future CDPK research.
出处
《草业学报》
CSCD
北大核心
2018年第1期204-214,共11页
Acta Prataculturae Sinica
基金
内蒙古科技计划项目(20140707)
国家自然科学基金青年科学基金项目(31502004)资助
