摘要
以来自世界各地的167份西瓜种质资源为材料,利用形态学标记和SSR标记,结合多样性分析、聚类分析和主坐标分析等方法,对其遗传多样性进行了系统全面的研究。结果表明,29个表型性状Shannon多样性指数的变化范围是0.54~2.03,平均值为1.50,其中质量性状平均值为1.16,数量性状为1.71;18个数量性状的变异系数变化范围为16.43%~90.73%,平均值为34.12%。不同地区西瓜种质遗传多样性比较结果显示,非洲地区的种质遗传多样性丰富,亚洲、北美洲次之,南美洲和欧洲的遗传多样性相对较低。同时,利用22对SSR标记对西瓜种质进行了遗传多样性分析,平均每对引物扩增4.86个等位基因,平均有效等位基因数(Ne)是2.20,平均Shannon多样性指数(I)为0.94,平均观测杂合度(Ho)为0.27,平均期望杂合度(He)为0.52,平均多态性信息含量(PIC)为0.45。进一步分别利用表型标记和SSR标记对167份西瓜种质材料进行分类,结果显示SSR标记的稳定性强,能够很好的对种质材料进行分类。
In this study, the genetic diversity of 167 watermelon germplasms, which were collected from different regions all over the world, were revealed by morphological markers and SSR markers, respectively. Twenty nine watermelon morphological characters were investigated and their Shannon information indexes （I） were ranged from 0.54 to 2.03 with a mean of 1.50. The mean values for quality characters and quantitative characters were 1.16, and 1.71, respectively. The range of variation coefficient was from 16.43% to 90.73%, and the average variation coefficient was 34.12%. Furthermore, 22 SSR markers were selected for genetic diversity analysis in 167 watermelon materials. The average alleles detected for each primer was 4.86. The average number of alleles （Ne） was 2.20. The average Shannon＇ s information index （I） for each primer was 0.94. The average observed heterozygosity （Ho） and expected heterozygosity （He） for each primer were 0.27 and 0.52, respectively. The average polymorphism information content （PIC） was 0.45. The results of phylogenetic analysis revealed that compared to morphological markers, SSR markers are more reliable and more suitable for being used in genetic diversity analysis.
出处
《中国瓜菜》
CAS
北大核心
2018年第1期5-11,共7页
China Cucurbits And Vegetables
基金
河南农业大学科技创新基金(KJCX2015A11)
关键词
西瓜
遗传多样性
形态学标记
SSR标记
Watermelon
Genetic diversity
Morphological markers
SSR markers
