摘要
目的观察丙烯酰胺(ACR)对幼年大鼠学习记忆能力的影响及维生素C对ACR毒性的缓解作用。方法将40只SPF级SD大鼠随机分为对照组(生理盐水)和低剂量组(2.5 mg/kg)、高剂量组(10 mg/kg)和ACR+维生素C泡腾片(维生素C组,10 mg/kg ACR+1 mg/kg维生素C),每组10只,雌雄各半。采用灌胃方式进行染毒,每周染毒5次,连续染毒4周。每周测量大鼠体质量、染毒结束后进行转棒试验和水迷宫试验及大鼠脑组织HE染色。结果与对照组相比,高剂量ACR染毒可引起大鼠体质量减轻(P<0.05)、转棒停留时间缩短(P<0.05)、水迷宫试验潜伏期延长(P<0.05)和游泳路程增加(P<0.05);HE染色提示大鼠海马组织有损伤;维生素C组的各项指标好于实验组。结论ACR可引起幼年大鼠运动功能和学习记忆能力降低,维生素C可在一定程度上减轻ACR的毒性、缓解ACR对大鼠海马引起的损伤。
Objective To study the effects of acrylamide on learning and memory ability in young rats and alleviated effect of vitamin C. Methods Forty SPF grade SD rats weighing 70-90 g were randomly divided into four groups, 10 in each group and male and female in half. The control groups were given normal saline. The rats in experimental groups were given acrylamide dissolved in normal saline, at the doses of 2.5 mg/kg, 10 mg/kg and vitamin C group was given 10 mg/kg and 1 mg/kg vitamin at the same time. All animals were given test substances through oral gavage, 5 days/week, for 4 weeks. Body weight was measured per week. Special tests included the rotarod Test and the staining of rat brain tissue. Learning and memory ability was tested by Morris water maze （MWM）. Results Compared with the control group, the weight of rats in high-dose group treated with acrylamide was decreased （P〈0.05） and time for rats standing rotarod was decreased （P〈0.05） and the latency was extended and the distance was increased by Morris water maze （MWM）; The HE staining suggested that the hippocampal tissue of rats was damaged. The indexes of vitamin C group were better than the experimental groups. Conclusion Acrylamide can damage motor function and learning-memory capability in young rats and vitamin C can decrease the ACR toxicity and alleviate the hippocampal tissue damage in some degree.
出处
《实验动物与比较医学》
CAS
2017年第6期460-464,共5页
Laboratory Animal and Comparative Medicine
