摘要
本文利用计算机精子辅助分析仪和姬姆萨染色方法评价无卵黄稀释液OPTIXcell和有卵黄稀释液Optidyl冷冻保存湖羊、白头杜泊、黑头杜泊和澳洲白绵羊精液的效果。选用湖羊、白头杜泊、黑头杜泊和澳洲白绵羊的种公羊各5只,通过假阴道方法采集精液,合格精液分别用OPTIXcell和Optidyl稀释液稀释并冷冻。结果表明:Optidyl冷冻保存白头杜泊羊和澳洲白绵羊精液的精子在活精子比例(MR)、直线速度(VSL)、平均路径速度(VAP)、曲线轨迹的直线性(LIN)、空间平均路径的直线性(STR)和尾部鞭打频率(BCF)上优于OPTIXcell,同样的,湖羊在MR、曲线速度(VCL)、精子头侧摆幅度(ALH)、VSL和VAP上高于OPTIXcell无卵黄稀释液,而黑头杜泊羊使用OPTIXcell在MR、VSL、VCL、ALH和BCF的平均值上效果更好。无论哪个品种使用Optidyl有卵黄稀释液,其绵羊精子畸形率的比例都低于使用OPTIXcell无卵黄稀释液。综上,冷冻保存湖羊、白头杜泊羊、澳洲白绵羊精液适合采用Optidyl,而黑头杜泊羊精液适合采用OPTIXcell。
This experiment was conducted to evaluate the effect of OPTIXcell without yolk and Optidyl with yolk on the frozen semen quality of Hu-sheep, White dorper sheep, Black dorper sheep and Australian white sheep sperm by using computer assisted sperm analyzer and Giemsa stain method. Choose 5 kinds of male sheep from Hu-sheep, White dorper sheep, Black dorper sheep and Australian white sheep, collect semen through the method of artificial vagina. The results showed that to compare the effect of two diluents on the same variety sheep semen, the MR, VSL, VAP, LIN, STR and BCF parameter of White dorper sheep and Australian white sheep semen diluted by Optidyl were better than that of OPTIXcell, and the MR, VCL, VAP and ALH parameters of Hu-sheep semen diluted by Optidyl were higher than that of OPTIXcell, but the MR, VSL, VCL, ALH and BCF of Black dorper sheep semen diluted by OPTIXcel were better that of Optidyl. The rates of sperm deformity in all four sheep varieties semen diluted by Optidyl were lower than that of OPTIXcell. In conclusion, Optidyl was suitable for the semen of Hu-sheep, White duper sheep and Australian white sheep, while OPTIXcell was suitable for the semen of Black dorper sheep.
出处
《中国畜牧杂志》
CAS
北大核心
2018年第1期65-68,72,共5页
Chinese Journal of Animal Science
基金
河北农业大学青年学术带头人培养计划
优质种用肉羊快速繁育技术中试与示范(16826618D)
羔羊肉生产关键技术推广(16226608D)
