摘要
采用LED精量调制光源,以番茄品种‘Micro-Tom’为试材,设置红光(R)、蓝光(B)和红蓝组合光3︰1(3R1B)3个处理,以白光(W)为对照,研究红蓝光质对转色期间番茄果实主要品质及番茄红素生物合成关键酶基因表达的影响。结果表明,与对照相比,红光和3R1B处理可显著提高番茄果实可溶性糖含量和糖酸比,降低可滴定酸含量;蓝光下维生素C、可溶性蛋白和可滴定酸含量明显升高,可溶性糖含量较低,但与对照差异不明显。随着果实成熟,红光和3R1B处理下番茄红素含量及牻牛儿基牻牛儿基焦磷酸合成酶(GGPS)和八氢番茄红素合成酶(PSY)基因的表达量显著高于蓝光和对照,尤以3R1B处理最明显;蓝光则显著降低了番茄红素含量,GGPS和PSY1表达受到抑制。综上,蓝光能够促进维生素C、蛋白质和有机酸含量增加;红光和红蓝组合光则有利于碳水化合物的积累,且通过提高番茄红素生物合成关键酶基因GGPS和PSY1的转录水平和活性,促进番茄红素合成,尤以3R1B处理最佳。说明适宜比例的红蓝组合光有利于番茄果实转色,提高果实品质。
The LED as precision modulation light sources were used with 3 treatments including red（R）light,blue（B）light and combination of red and blue light with proportion of 3︰1（3 R1 B）,white light as control. The effects of red and blue light qualities on main fruit quality and gene expression of key enzymes involved in the biosynthesis of lycopene of tomato with ＇Micro-Tom＇as experiment material during color-turning period were studied. The results showed that compared with control,red and 3 R1 B light treatment could significantly increase the soluble sugar content and ratio of sugar and acid in tomato fruit,but reduced the titratable acid content. The content of vitamin C,soluble protein and titratable acid under blue light was significantly enhanced,while lower in the soluble sugar content and no significant difference was found compared with control. With the fruit maturation,the lycopene content and gene expression of geranylgeranyl pyrophosphate synthase（GGPS）and phytoene synthase（PSY）were significantly higher under red and 3 R1 B light than those under blue and white light,especially for 3 R1 B. Furthermore,the lycopene content was significantly reduced and the expression of GGPS and PSY1 was inhibited by blue light. In summary,blue light could promote the increment in levels of vitamin C,protein and organic acids. Red and combination of red and blue light were more conducive to the accumulation of carbohydrates,and promoted lycopene synthesis by enhancing the transcription levels and activities of GGPS and PSY1,which involved in the biosynthesis of lycopene,especially the latter treatment. It indicated that the combination of red and blue light with appropriate proportion could promote the color-turning of tomato fruit and improve fruit quality.
出处
《园艺学报》
CAS
CSCD
北大核心
2017年第12期2372-2382,共11页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31401921)
山东省自然科学基金项目(ZR2014CQ029)
山东农业大学设施园艺优势团队项目(SYL2017YSTD07)
"十二五"国家科技支撑计划课题(2014BAD05B03
2012BAD11B01)
国家现代农业产业技术体系建设专项资金项目(CARS-25)
"十三五"国家重点研发计划项目(2016YFB0302403)
国家公益性行业(农业)科研专项(201303108)
关键词
番茄
番茄红素
发光二极管
光质
果实品质
tomato
lycopene
light-emitting-diode
light quality
fruit quality
