摘要
目的:探讨无症状生殖道感染的不育男性精液中白细胞亚群与精子DNA损伤的相关性。方法:选择2013年3月至2015年8月就诊的无症状生殖道感染的不育男性111例,精液常规分析后,采用免疫细胞化学法检测精液中CD45^+白细胞浓度,流式细胞术检测单核细胞/巨噬细胞系CD14抗原^+、活化巨噬细胞HLA-DR^+细胞浓度;TUNEL法检测精子DNA碎片及8-羟化脱氧鸟苷(8-OHd G)表达情况;分析精液白细胞亚群与精子DNA损伤及常规精液参数的相关性。结果:精液CD45^+细胞浓度与CD14^+、HLA-DR^+细胞浓度间有明显相关性(P<0.01),而不同亚群白细胞浓度与精液传统参数、碎片率、8-OHd G^+细胞比例均无明显相关性。8-OHd G^+精子百分率与精子碎片百分率呈正相关(r=0.48,P<0.01),而与精子浓度呈负相关(r=-0.44,P<0.01)。在调整年龄、禁欲时间和吸烟的基础上,精子浓度和精子碎片率(β=-0.25,P=0.008;β=0.23,P=0.05)与8-OHd G^+精子百分率的变化独立相关。结论:无症状生殖道感染的不育男性精子DNA损伤与精液质量下降相关,而与不同白细胞亚群无相关性。
Objective ： To study the correlation of leukocyte subsets with sperm DNA damage in the semen of infertile men with asymptomatic genital tract infection （AGTI）. Methods： This study included 111 infertile males with AGTI. After routine semen analysis, we determined the concentration of CD45 ＋ leukocytes in the semen by immunocytochemistry, measured the concentrations of CD14＋ cells of the mononuclear / maerophagic system and activated macrophages and HLA-DR ＋ cells in the semen by flow cytometry, and examined the sperm DNA fragmentation index （DFI） and the expression of 8-hydroxy-2＂-deoxyguanosine （8-OHdG） by TUNEL as- say. Then we analyzed the correlation of seminal leukocyte subsets with sperm DNA damage and routine semen parameters. Results ： The concentration of CD45 ＋ leukocytes was correlated significantly with those of CD14 ＋ and HLA-DR ＋ cells in the semen （P 〈0. 01 ） , but not that of leukocyte subsets with routine semen parameters, sperm DFI, or the percentage of 8-OHdG-positive cells. Thepercentage of 8-OHdG-positive sperm was correlated positively with the sperm DFI （ r = 0.48, P 〈 0.01 ） but negatively with sperm concentration （r = -0.44, P 〈0.01 ）. After adjusted for age, abstinence time and cigarette smoking, the percentage of 8-OHdG- positive sperm was correlated independently with sperm concentration （ [3 = - 0.25, P = 0. 008 ） and DFI （ β = 0.23, P = 0.05）. Conclusion： Sperm DNA damage is associated with poor semen quality but not with seminal leukocyte subsets in infertile males with asymotomatic genital tract infection. Natl .I Androl.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2018年第1期45-49,共5页
National Journal of Andrology
