摘要
为了了解我国不同地区规模化猪场2014年以来猪伪狂犬病病毒（PRV）流行株的现状、分子生物学特点和遗传演化规律，本试验从我国15个省份不同地区分离得到29株PRV，并对其gB，gC和gE基因进行遗传变异分析。结果显示，这29株PRV分离株主要为PRV变异毒株，与Bartha株等国外经典毒株亲缘关系较远，与Ea株等国内经典毒株亲缘关系较近。氨基酸序列比对发现，29株PRV变异毒株在gB，gC及gE基因上均发生了多个位点的突变：gB氨基酸序列均存在75S、76P和77G的连续缺失，94位点均存在甘氨酸（G）的插入；gC氨基酸序列存在多处改变，其中P87Q和Y142C使gc糖蛋白与宿主细胞表面的硫酸乙酰肝素受体结合功能域发生改变；gE氨基酸序列第48位点均存在天冬氨酸（D）的插入。这些特征性变异位点可作为分子诊断依据，并且从分子水平揭示了临床Bar—tha—K61株等经典毒株疫苗免疫效果下降的可能原因。
To study the genetic diversity, molecular epidemiology and phylogenetic relationship of epidemic PRV strains,phylogenetic analysis based on partial gE, gB and gC genes of twenty-nine PRV strains which were isolated from fifteen provinces around China were carried out. The results showed that the 29 PRV isolates were mainly PRV variants,and the relationship between the 29 PRV isolates and Ea strain was closer than that of Bartha strain. Meanwhile,amino acid analysis based on partial gB and gE gene and complete gC gene revealed multiple mutation sites comparing the isolated PRVs with Bartha-K16. The 75S,76P and 77G gene serial deletion was displayed in the amino acids of gB,in addition to the insertion of glycine on the 94th amino acid. For gene gC,P87Q and Y142C disrupted the functional binding binding domain of the heparin sulfate on the serted with asparagine, which suggested that nosis,but the relationship between these m polymorphic sites characteristics can be used possible reasons for the decline of the immun from the molecular level. domain between gC glycoprotein and the receptor host cell surface. For gene gE,the 48th site was in- these mutation site could be used for molecular diagutation and its pathogenicity was undefined. These as the basis for molecular diagnosis, and reveal the e effect of classical strains such as Bartha-K61 strains
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第1期1-10,共10页
Chinese Journal of Veterinary Science
基金
口蹄疫等重要家畜疫病防控净化技术集成研究与示范课题资助项目(2015BAD12B04)
优良种猪高效利用关键工程化技术集成与示范课题资助项目(2014BAD20B00)
关键词
伪狂犬病病毒
序列分析
分子特征
变异毒株
Pseudorabies virus
sequencing analysis
molecular characteristics
variant strain
