摘要
为研究二元双水相体系对欧李种仁清蛋白和球蛋白的萃取条件,采用(乙醇+丙酮)(二者体积比为1∶2)/硫酸铵构成的二元双水相体系,对欧李种仁的清蛋白和球蛋白分别进行萃取,通过比较2种蛋白在此二元双水相体系中的分配系数、回收率等特性,最终确定欧李种仁清蛋白和球蛋白的最佳萃取条件。实验结果表明:当二元双水相体系组成为27.5%(乙醇+丙酮)(二者体积比为1∶2)和26%硫酸铵时,清蛋白的分配系数最小,萃取率达到最大;当二元体系组成为17.5%(乙醇+丙酮)(二者体积比为1∶2)和24%硫酸铵时,球蛋白的分配系数最小,萃取率达到最大。二元双水相体系为分离清蛋白与球蛋白提供了新的途径,在植物来源的清蛋白与球蛋白提取中具有良好的应用前景。
In order to optimize the extraction conditions,an binary aqueous two-phase system(BATPS)composed of ethanol and acetone(volume ratio is 1∶2)and ammonium sulphate is selected to extract globulin and albumin from Cerasus humilis seeds.The distribution characteristic of the globulin and albumin in this aqueous two-phase system is reflected by comparing critical data such as partition coefficient and recovery rate.The experiment shows that the optimum conditions of albumin is composed of 27.5% ethanol and acetone(volume ratio is 1∶2),and 26% ammonium sulphate,the optimum conditions of globulin is composed of 17.5%ethanol and acetone(volume ratio is 1∶2),and 24%ammonium sulphate.These results suggest that the BATPS is efficient in extracting albumin and globulin with bright application prospect,and has great potential to beused in natural protein extraction.
出处
《河北科技大学学报》
CAS
2018年第1期35-41,共7页
Journal of Hebei University of Science and Technology
基金
四川省高等学校重点实验室开放基金(10Y201409)
关键词
生物工程其他学科
二元双水相体系
萃取
球蛋白
清蛋白
other disciplines of bioengineering
binary aqueous two-phase system(BATPS)
extraction
globulin
albumin
