摘要
目的观察醒鼻温敏凝胶剂对变应性鼻炎(AR)大鼠血清白细胞介素-4(IL-4)、转化生长因子-β1(TGF-β1)及鼻黏膜嗜酸性粒细胞(EOS)计数的影响,探讨其作用机制。方法以卵清白蛋白加氢氧化铝腹腔注射基础致敏,局部以2%卵清白蛋白激发成功复制AR模型。实验大鼠随机分为正常组、模型组、阳性对照组和醒鼻低、中、高剂量组,各给药组给予相应药物干预。比较各组大鼠症状评分,检测血清IL-4、TGF-β1含量和鼻黏膜EOS计数。结果与正常组比较,模型组大鼠血清IL-4、TGF-β1含量明显升高(P<0.01),鼻黏膜组织EOS计数明显增加(P<0.05);与模型组比较,醒鼻中剂量组和阳性对照组血清IL-4、TGF-β1含量明显降低(P<0.01),鼻黏膜组织EOS计数明显减少(P<0.05)。结论醒鼻温敏凝胶剂可能通过下调IL-4、TGF-β1等炎症因子水平及减少鼻黏膜EOS浸润而发挥治疗作用。
Objective To observe the effects of Xingbi Wenmin Gel on serum levels of IL-4, TGF-β1 and nasal mucosa eosinophils （EOS） in allergic rhinitis （AR） rats; To discuss its mechanism of action. Methods Intraperitoneal injection of egg albumin and aluminum hydroxide based sensitized to ovalbumin challenge 2% local AR model was established successfully. The experimental rats were randomly divided into normal group, model group, positive control group, and Xingbi Gel low-, medium-, and high-dosage groups. Each medication group was given relevant medicine for intervention. The rat symptom scores in each group were compared, and serum IL-4, TGF-β1, and nasal mucosa EOS counts were detected. Results Compared with the normal group, the serum levels of IL-4 and TGF-β1 in model group increased significantly （P〈0.01）, and the nasal mucosa EOS counts increased significantly （P〈0.05）. Compared with model group, the serum levels of IL-4 and TGF-β1 in Xingbi Gel medium-dosage group and positive control group decreased significantly （P〈0.01）, and the nasal mucosa EOS counts decreased significantly （P〈0.05）. Conclusion Xingbi Wenmin Gel may be effective through down-regulation of IL-4, TGF-β1 levels of inflammatory factors, and reduction of EOS infiltration in nasal mucosa.
出处
《中国中医药信息杂志》
CAS
CSCD
2018年第2期47-50,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81373820)
福建省自然科学基金(2015J01683)
