摘要
目的探讨构建兔急性阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OSAHS)并发心房颤动模型的可行性。方法 10只新西兰大白兔,麻醉后开胸,于呼气末时夹闭气管导管诱导OSAHS模型,并通过程序刺激(S1S2)诱导心房颤动模型,观察造模前,造模后第1、2、4h气管导管夹闭前、气管导管夹闭后股动脉pH值、pa(CO_2)、pa(O_2)及观察造模前,造模后第1、2、3、4h时右心房有效不应期和心房颤动持续时间。结果造模前,造模后第1、2、4h气管导管夹闭后兔动脉血pH(7.32±0.01、7.35±0.06、7.32±0.02、7.28±0.07)、pa(O_2)[(4.81±0.30)、(4.70±0.24)、(4.41±0.23)、(3.98±0.34)/kPa]均低于气管导管夹闭前[7.43±0.03、7.44±0.07、7.43±0.05、7.42±0.04;(12.19±0.27)、(12.30±0.27)、(12.39±0.79)、(12.32±0.17)kPa],pa(CO_2)[(5.62±0.26)、(5.58±0.66)、(5.61±0.43)、(5.77±0.38)kPa]高于气管导管夹闭前[(4.01±0.41)、(3.99±0.21)、(3.85±0.37)、(3.79±0.34)kPa](P<0.05);不同时间点气管导管夹闭前及气管导管夹闭后兔动脉血pH、pa(O_2)、pa(CO_2)比较差异均无统计学意义(P>0.05);随OSAHS造模时间延长,右心房有效不应期逐渐缩短,心房颤动持续时间逐渐延长,且均在造模2h时差异开始有统计学意义(P<0.05)。结论通过气管导管夹闭联合程序刺激可成功构建OSAHS并发心房颤动兔模型,且模型稳定,操作相对简单,具有可行性。
Objective To investigate the feasibility of the establishment of rabbit models with atrial fibrillation complicated with acute obstructive sleep apnea hypopnea syndrome （OSAHS）. Methods Ten New Zealaad white rabbits underwent tboracotomy under anesthesia, and OSAHS models were induced by clipping tracheal tube at the end of expiration followed by the establishment of atrial fibrillation models by program stimulation （$1S2） . The femoral artery pH value, pa（CO2 ） and pa（O2） before and in 1, 2 and 4 h after clipping tracheal tube, and effective refractory period and AF lasting time of right atrium before and in 1, 2, 3 and 4 h after modeling were detected. Results The arterial blood pH values （7.32±0.01, 7.35±0. 06, 7. 32±0. 02, 7. 28±0. 07） and pa（O2） values （（4.81±0.30）, （4.70±0. 24）, （4.41±0.23）, （3.98± 0.34） kPa） before and 1, 2 and 4 h after modeling were significantly lower than those before clipping tracheal tube （7.43±0.03, 7.44±0.07, 7.43!0.05, 7.42±-_0.04± （12.19±0.27）, （12.30＋_0.27）, （12.39± 0.79）, （12.32±0.17） kPa）, and pa（CO2） values （（5.62±0.26）, （5.58±0.66）, （5.61±0.43）, （5.77±0.38） kPa） were significantly higher than those before clipping tracheal tube （（4. 01 ± 0. 41）, （3. 99± 0. 21）, （3. 85 ± 0. 37）, （3.79±0.34） kPa） （P〈0.05）. There were no significant dif{erences in femoral artery pH values, pa（CO2 ） values and pa（O2 ） at different time points of clipping tracheal tube （P±0.05）. With the prolongation of OSAHS modeling time, the effective refractory period was gradually shortened and the duration of atrial fibrillation gradually extended, which began to show differences in 2 h after modeling （P±0.05）. Conclusion Rabbit models with OSAHS and atrial fibrillation can he successfully established by clipping tracheal tube plus program stimulation, and the model is stable, with relative simple facility and feasible method.
出处
《中华实用诊断与治疗杂志》
2018年第1期10-13,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
国家自然科学基金(81560064)
新疆自治区青年博士科技人才项目(qn2015bs009)
