摘要
目的:探讨加味小柴胡汤治疗慢性乙型肝炎(CHB)的可能作用机制。方法:临床CHB肝郁脾虚证患者随机分为中药治疗组、西药治疗组,各30例,分别予加味小柴胡汤、恩替卡韦治疗7 d后收集含药血清,另设健康组30例取血清,将上述人血清分别作用于HepG2.2.15细胞中药组(10%中药组、20%中药组)、西药组、对照组,在48、72、144 h 3个时间节点收集细胞与上清,用ELISA法检测细胞上清HBs Ag含量;实时荧光定量PCR法检测细胞上清HBV DNA含量及细胞JAK2、STAT3 mRNA表达水平。结果:经过干预,10%中药组、20%中药组HBs Ag在干预48、96、144 h后明显下降(P〈0.01)。干预48 h、96 h后,20%中药组STAT3 mRNA表达水平较对照组、西药组明显升高(P〈0.01,P〈0.05)。结论:加味小柴胡汤可抑制细胞内HBV,上调STAT3的表达,这可能是其治疗CHB的作用机制之一。
Objective:To explore the underlying possible mechanism of Jiawei Xiaochaihu Decoction( JXD) in treating chronic hepatitis B( CHB). Methods:The CHB patients with liver stagnation and spleen deficiency syndrome were randomly assigned into Chinese or Western medicine treatment group,30 cases in each group,collecting the medicated serum after treatment with JXD or entecavir for 7 days. Healthy group had 30 cases. The sera of three groups above was added to the HepG2. 2. 15 cell groups. Chinese medicine treatment( including10% and 20% Chinese medicine treatment),Western medicine treatment and healthy respectively. Cells and supernatant were collected at three time nodes(48 h,96 h and 144 h). The expression of HBs Ag in the cell supernatant was detected by ELISA,HBV DNA in supernatant,JAK2 and STAT3 mRNA in cells by Real-time quantitative PCR. Results:The expression of HBs Ag in the groups of 10% and 20%Chinese medicine treatment significantly decreased after intervention at 48,96,144 h time node( P 〈 0. 01). The expression of STAT3 mRNA in the group of 20% Chinese medicine treatment was significantly higher than that of control and western medicine treatment group at the time node of 48 h and 96 h( P 〈 0. 01,P 〈 0. 05). Conclusion: JXD could suppress HBV and up-regulate the expression of STAT3,which might be one of its mechanisms in treating chronic hepatitis B.
出处
《中华中医药学刊》
CAS
北大核心
2018年第1期38-41,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81403331)
福建省自然科学基金项目(2014J01363)