摘要
为有效抑制稻梨孢菌对水稻的侵染,以2株稻梨孢菌37631、37661为供试菌株,采用生长速率测定法对5种植物生长调节剂(油菜素内酯、生长素、细胞分裂素、赤霉素和脱落酸)进行了抑菌活性测定,并研究了油菜素内酯对稻梨孢菌菌丝体干质量和孢子萌发的影响。结果显示,油菜素内酯对稻梨孢菌菌丝延伸具有明显的抑制作用,当浓度达到5μmol/L时,其对2个菌株的菌丝延伸抑制率分别为58.18%和53.37%,半数效应浓度(EC_(50))分别为3.7μmol/L和5.2μmol/L;同时,油菜素内酯处理可在一定程度上改变菌落形态。其他4种植物生长调节剂对稻梨孢菌菌丝延伸没有明显的抑制作用。菌丝干质量测定结果显示,油菜素内酯能明显抑制稻梨孢菌的生长,在2μmol/L处理情况下,培养至第8天时2株稻梨孢菌菌丝干质量与对照(0μmol/L处理)相比分别减少了52.60%和48.44%。通过对稻梨孢菌孢子萌发的研究发现,油菜素内酯对稻梨孢菌的孢子萌发也具有明显的抑制作用,当浓度达到5μmol/L时,其对2个菌株的孢子萌发抑制率分别为53.25%和49.34%,EC_(50)值分别为4.31μmol/L和6.42μmol/L。综上,油菜素内酯对稻梨孢菌的菌丝延伸、菌丝干质量及孢子萌发都有明显影响。
To inhibit the infection of rice by Pyricularia oryzae effectively,the antimicrobial activity of five plant growth regulators( brassinosteroid,auxin,cytokinin,gibberellin and abscisic acid) was determined by growth rate method using two strains of Pyricularia oryzae( 37631,37661),and the effect of brassinosteroid on hypha dry weight and spore germination was studied. The results showed that epi-brassinolide( epi-BL) had a significant inhibitory effect on mycelial elongation of Pyricularia oryzae. When the concentration of epi-BL reached 5 μmol/L,the inhibitory rates on mycelial elongation of two strains were 58. 18% and 53. 37%,and the EC_(50) values were 3. 7 μmol/L and 5. 2 μmol/L,respectively. At the same time,the colon morphology of two strains was found to be changed after epi-BL treatment. The mycelial elongation of two strains was not significantly inhibited by other four plant growth regulators. The result of hypha dry weight experiment showed that,compared with the control( 0 μmol/L epi-BL),the dry weights of two strains were reduced by 52. 60% and 48. 44%,respectively,after 8 days of 2 μmol/L epi-BL treatment. epi-BL also had significant inhibitory effect on the spore germination of Pyricularia oryzae. Whenthe concentration reached 5 μmol/L,the inhibition rates on the two strains were 53. 25% and 49. 34%,and the EC_(50) values were 4. 31 μmol/L and 6. 42 μmol/L,respectively. Overall,it can be found that the mycelium elongation,dry weight and spore germination of Pyricularia oryzae are significantly influenced by brassinosteroid.
出处
《河南农业科学》
CSCD
北大核心
2017年第12期70-74,共5页
Journal of Henan Agricultural Sciences
基金
陕西理工大学人才启动项目(SLGKYQD2-19)
陕西省科技厅重点实验室项目(2015SZS-15-07)
陕西省教育厅重点科学研究计划项目(15JS021)
关键词
稻梨孢菌
植物生长调节剂
油菜素内酯
抑菌活性
菌丝生长
孢子萌发
Pyricularia oryzae
plant growth regulator
brassinosteroid
antimicrobial activity
mycelium growth
spore germination
