摘要
以橡胶籽为原料,研究了橡胶籽β-葡萄糖苷酶的热稳定性,以及在室温和高温下(105℃、110℃和115℃),不同压力条件对橡胶籽粉、橡胶籽冻干粉β-葡萄糖苷酶活性的影响。结果表明:橡胶籽β-葡萄糖苷酶热稳定性的边界温度在90℃到100℃之间,冻干粉为100℃~110℃;室温下,橡胶籽粉中β-葡萄糖苷酶活性随着压力增大,呈现先下降后上升又下降的趋势。压力对橡胶籽冻干粉β-葡萄糖苷酶有明显的激活作用,在压力为30 MPa时,激活作用最为明显;高温处理后(0 MPa),冻干粉酶活明显高于橡胶籽粉;105℃加压处理橡胶籽粉和冻干粉酶活变化趋势接近,为先上升后下降又上升,压力对橡胶籽粉的作用效果更为明显;110℃加压处理,在0~20 MPa内都酶活呈现先上升后保持的趋势,橡胶籽粉在20 MPa以后为先下降后上升而冻干粉酶活则一直上升;115℃加压处理时,橡胶籽粉β-葡萄糖苷酶酶活一直处于上升状态,而冻干粉在10~20 MPa出现了酶活的降低,后又恢复上升趋势。
In this paper, Hevea brasiliensis seeds were used as materials to study the thermal stability offl-glucosidase. And the influence of different stress condition on the β-ghicosidase of Hevea brasiliensis seeds powder and Hevea brasiliensis seeds powder after freeze drying at room temperature and high temperature （105 22, 1 10 22, 1 15 22 ）. The results showed that the thermal stability boundary temperature of β-glucosidase ofHevea brasiliensis seeds powder was between 90 22 to 100 22, lyophilized powder was between 100 22 to 110 22; At room temperature, with the increase of pressure, the activity of fl-glucosidase of Hevea brasiliensis seeds powder decreased, and then showed a tendency to decrease after increasing. The activation effect was most obvious when the pressure was 30 MPa. After heart treatment （0 MPa）, the activity of lyophilized powder was significantly higher than that of Hevea brasiliensis seeds powder. The change of enzyme activity of 105 22 pressurized Hevea brasiliensis seeds powder and lyophilized powder had similiar trendency, showed a tendency to rise after decreasing and rising again, the effect of pressure on the Hevea brasiliensis seeds powder was more obvious; 110 22 pressure treatment, the enzyme activity in 0-20 MPa showed a trendency to remain unchanged after rising, the Hevea brasiliensis seeds powder increased after 20 MPa after the first decrease, and the activity of β-glucosidase of lyophilized powder increased all the time; 1 1 5 22 pressure treatment, the activity of β-glucosidase of the Hevea brasiliensis seeds powder had been increasing, while the enzyme activity of lyophilized powder was reduced in 10-20 MPa, and then rised again.
出处
《食品工业》
CAS
北大核心
2018年第1期26-30,共5页
The Food Industry
基金
国家自然科学基金(31460407)
关键词
高压
橡胶籽
Β-葡萄糖苷酶
high pressure
Hevea brasiliensis seeds
β-glucosidase
