摘要
目的观察"通督启神"法脉冲电针与音乐电针对APP/PS1模型小鼠空间学习记忆能力及海马内炎症介质表达的影响。方法 APP/PS1双转基因雄性小鼠32只随机分为模型组(n=8)、药物组(n=8)、脉冲电针组(n=8)和音乐电针组(n=8),相同月龄C57BL/6雄性小鼠作为正常对照组(n=8)。脉冲电针组与音乐电针组以各自电针仪电针百会、印堂,留针20 min,取针后点刺人中。药物组予盐酸多奈哌齐0.92 mg/kg灌胃。正常对照组、模型组与药物组以相同方法抓取束缚20 min。治疗15 d后,行Morris水迷宫测试,免疫组化观察海马高迁移率族蛋白B1(HMGB1)与白细胞介素-10(IL-10)表达,Western blotting检测海马HMGB1与IL-10的表达。结果两个电针组从训练第4天起,逃避潜伏较模型组缩短(P<0.05),音乐电针组最短;目标象限游泳比例升高(P<0.05),两个电针组之间无显著性差异(P>0.05);海马HMGB1的表达降低(P<0.05),IL-10蛋白表达增加(P<0.05),免疫组化法音乐电针组HMGB1的表达低于脉冲电针组(P<0.05),IL-10表达高于脉冲电针组(P<0.05),Western blotting检测两组无显著性差异(P>0.05)。结论 "通督启神"法脉冲电针与音乐电针均能改善APP/PS1小鼠学习记忆功能,音乐电针在抑制炎性因子表达、促进抗炎因子表达方面可能优于脉冲电针。
Objective To compare the effect of impulse electroacupuncture(impulse-EA) and music electroacupuncture(music-EA)for Tongdu Qishen on spatial learning and memory, and expression of cytokine in the hippocampus of APP/PS1 mice.Methods A total of 32 APP/PS1 double transgenic male mice were randomly divided into model group(n=8), drug group(n=8), impulse-EA group(n=8) and music-EA group(n=8), the same background and age male C57 BL/6 mice were observed as normal group(n=8). The impulse-EA group and music-EA group accepted EA at Baihui(GV20) and Yintang(GV29), connected with their own electroacupuncture stimulators, for 20 minuts, then, they were pricked Renzhong(GV26) for a while. The drug group accepted donepezil hydrochloride 0.92 mg/kg intra-gastrically. The normal group, model group and drug group were grabbed and bounded in the same way. After 15 days of treatment, they were assessed with Morris water maze. The expression of high mobility group box protein-1(HMGB1) and interleukin-10(IL-10) in hippocampus were measured with immunohistochemistry and Western blotting.Results The escape latency shortened in EA groups compared with that of the model group since the fourth day of Morris water maze training(P<0.05), and it was the least in the music-EA group; and the ratio of swimming across the target quadrant increased(P<0.05), but there was no significant difference between EA groups(P>0.05). The expression of HMGB1 decreased(P<0.05) and the expression of IL-10 increased(P<0.05) in EA groups compared with that of the model group, and HMGB1 decreased more and IL-10 increased more in the music-EA group than in the impulse-EA group(P<0.05), according to the measurement in immunohistochemistry, not in Western blotting.Conclusion Both impulse-EA and music-EA based on Tongdu Qishen can promote the recovery of the learning and memory in APP/PS1 mice, and music-EA may do more effectively in inhibition of pro-inflammatory factors and promotion of the anti-inflammatory factors.
出处
《中国康复理论与实践》
CSCD
北大核心
2018年第1期17-22,共6页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金面上项目(No.81473774)~~
