摘要
目的:通过比较分析应激状态下高血压肝火亢盛证大鼠的宏观表征及微观理化指标的变化,探讨肝主疏泄的生物学基础。方法:模拟现代社会的应激刺激,给予高血压肝火亢盛证大鼠和正常Wistar大鼠相同的应激刺激,动态监测其血压、易激惹程度、行为及血液中Ang-Ⅱ、NE含量变化,进行多个维度的差异性及关联性分析。结果:高血压肝火亢盛证大鼠应激组与无应激组相比,应激组高血压肝火亢盛证大鼠血压升高幅度更大(P<0.05),易激惹程度评分更高(P<0.05),血液中Ang-Ⅱ、NE含量升高(P<0.05),且应激结束2周后各项指标仍然显著高于无应激组高血压肝火亢盛证大鼠(P<0.05),而Wistar应激组与无应激组相比,虽然也各项指标均有升高(P<0.05),但相较高血压肝火亢盛证大鼠较晚出现升高,且应激结束2周后与对照组无统计学意义(P>0.05)。结论:在肝疏泄功能正常的状态下,受到外界应激时,一定程度上机体可作出适应性反应,而高血压肝火亢盛证属肝失疏泄状态,应激刺激下出现应激损伤表现,其机制可能与外周Ang-Ⅱ、NE含量升高有关。
Objective： To observe the macro and physicochemical changes of hypertensive rats with live-fire hyperactivity syndrome under stress,to evaluate the effects of stress in the process of hypertension,expounds the theoretical basis of liver governing the free flow of qi. Methods： Stress with same intensity was given to hypertension rats with liver fire hyperactivity（ SHR 14-18 weeks old） and normal rats（ Wistar at the same age）. The blood pressure,irritability degree,behavior and content of Ang-Ⅱ and NE in serum were dynamically meassured. The difference and correlation for multiple dimensions were analyzed. Results： The adaptability to stress of hypertensive rats with liver-fire hyperactivity was worse than normal rats. Compared with non-stress group of SHR,the pressure increased significantly（ P〈0. 05）,and score of irritability degree was higher（ P〈0. 05）. The contents of Ang-Ⅱ and NE in the blood was increased（ P〈0. 05）,and 2 weeks after the stress stopped,the above indexes were still significantly higher than non-stress SHR（ P〈0. 05）. Although the indexes of normal Wistar Rats under stress increased（ P〈0. 05）,but compared with the SHR which appeared in the late stage of stress later,and the indicators returned to normal level 2 weeks after stress stopped,and there was no significant difference（ P〈0. 05）. Conclusion： In normal physiological conditions,liver function is normal. The body can make adaptive response if affected by external stress in a certain extent,but under dysfunction of the liver,stress injury will occur,the mechanism of which may be related to the content increase of NE and Ang-Ⅱ.
出处
《世界中医药》
CAS
2017年第12期3063-3067,共5页
World Chinese Medicine
基金
国家自然科学基金青年项目(81503382)
国家自然基金面上项目(81473521)
北京中医药大学中青年教师项目(面上项目)(2015-JYB-JSMS036)
国家重点基础研究发展计划(973计划)项目(2011CB505106)
关键词
高血压
肝火亢盛证
应激
肝主疏泄
生物学基础
Hypertension
Liver-fire hyperactivity syndrome
Stress
Liver governing the free flow of qi
Biological basis
