摘要
目的研究蓬乱蛋白2(Dvl2)在高脂血症大鼠种植体周围早期的表达。方法 24只雄性Wistar大鼠,随机均分为实验组和对照组,分别给予高脂和普通饲料。8周后,检测大鼠血脂水平并于股骨干骺端植入种植体,术后1、3、5 d处死,获得种植体周围骨组织。采用亚甲基蓝-酸性品红染色观察种植体-骨界面,采用实时荧光定量聚合酶链反应(RT-PCR)检测Runt相关转录因子2(Runx2)、组织蛋白酶K(Cat K)和Dvl2的表达,免疫印迹和免疫共沉淀法检测Dvl2、磷酸化Dvl2和泛素化Dvl2的表达。结果与对照组相比,实验组的成骨细胞、Runx2、Dvl2和磷酸化Dvl2减少(P<0.05),而破骨细胞、Cat K和泛素化Dvl2增多(P<0.05)。结论高脂血症可能通过上调Dvl2及磷酸化、下调泛素化而抑制种植体周围早期骨改建。
Objective This study aims to investigate the expression of disheveled 2 （Dvl2） around the implant of hyperli-pidemic rats at an early stage after the implantation. Methods A total of 24 Wistar rats were divided equally into the experi-mental group fed with high-fat diet group and control group fed with a normal diet. After 8 weeks, the serum lipid levels were detected, and rats received implants in the femur metaphysis. Rats were sacrificed at 1, 3, and 5 days after implantation, and the bones around implants were obtained. Methylene blue-acid fuchsin staining was performed to observe the implant-bone interface. Real-time polymerase chain reaction was performed on runt-related transcription factor 2 （Runx2）, cathepsin K （CatK）, and Dvl2. Dvl2 Western blot or immunoprecipitation, phosphorylation, and ubiquitination were also conducted. Results In the experimental group, less osteoblasts, lower expression of Runx2 and Dvl2, and lower Dvl2 phosphorylation （P〈0.05） than those of the control group were observed; furthermore, the CatK expression and Dvl2 ubiquitination were higher than those in the control group （P〈0.05）. Conclusion Hyperlipidemia may suppress bone remodeling around the implant at an early stage by Dvl2 down-regulation, phosphorylation, and up-regulated ubiquitination.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2018年第1期82-86,共5页
West China Journal of Stomatology
基金
国家自然科学基金(81671025)
山东省科技发展计划(2015GSF118186)
