摘要
目的探讨凋亡抑制蛋白(Survivin)、环氧合酶-2(COX-2)和P-糖蛋白(P-gp)在大肠癌中的表达,并分析其与临床病理特征的关系。方法采用免疫组化法(GTVision法)检测2014年1—12月该院60例结直肠腺癌组织和20例正常结直肠黏膜组织中Survivin、COX-2及P-gp的表达。结果 Survivin在结直肠癌组织中表达的阳性率为71.7%(43/60),在正常黏膜组织内为15.0%(3/20),二者比较,差异有统计学意义(P<0.01);COX-2在结直肠癌组织中表达的阳性率为66.7%(40/60),在正常黏膜组织内为20.0%(4/20),二者比较,差异有统计学意义(P<0.01);P-gp在结直肠癌组织中表达的阳性率为25.0%(15/60),在正常黏膜组织内为10.0%(2/20),二者比较,差异无统计学意义(P>0.05);Survivin和COX-2均与浸润深度、淋巴结转移、肿瘤分期相关(P<0.05),而与结直肠腺癌患者性别、年龄、肿瘤大小及分化程度无相关性(P>0.05);Survivin和COX-2蛋白表达呈正相关(r=0.418,P<0.01)。结论在结直肠癌中Survivin和COX-2蛋白呈高表达,P-gp呈低表达或无表达。Survivin和COX-2蛋白的高表达与大肠癌临床病理特征关系密切,且二者表达具有协同效应,协同促进大肠肿瘤的发生与发展。
Objective To explore the expression of Survivin,COX-2 and P-gp in colorectal cancer,and to analyze their relationship with clinicopathologic features.Methods The expression of Survivin,COX-2 and P-gp in 60 cases of colorectal adenocarcinoma tissue and 20 cases of normal colorectal mucosal tissue in this hospital from January to December2014 were detected by adopting the immunohistochemical method（GTVision method）. Results The positive expression rate of survivin was 71.7%（43/60）in colorectal cancer tissue and 15.0%（3/20）in normal mucosal tissue,the difference between two groups was statistically significant（P〈0.01）;the positive expression rate of COX-2 was 66.7%（40/60）in colorectal cancer and20.0%（4/20）in the normal mucosal tissue,the difference between two groups was statistically significant（P〈0.01）;the positive expression rate of P-gp was 25.0%（15/60）in colorectal cancer and 10.0%（2/20）in the normal mucosal tissue,the difference between two groups was not statistically significant（P〉0.05）;Survivin and COX-2 were correlated with the infiltration depth,lymph node metastasis and clinical stage（P〈0.05）,but had no correlation with the gender,age,tumor size and differentiation degree（P〉0.05）.The positive correlation was found between Survivin and COX-2 protein expression（r=0.418,P〈0.01）.Conclusion Survivin and COX-2 are highly expressed in colorectal cancer,and P-gp is lowly expressed or has no expression.The high expression of Survivin and COX-2 protein are closely related with the clinicopathological characteristics of colorectal cancer,moreover their expressions have synergistic effect and synergistically promote the occurrence and development of colorectal cancer.
出处
《现代医药卫生》
2018年第2期171-174,共4页
Journal of Modern Medicine & Health
基金
汇川区科技局项目(209.001.122.08)
