摘要
【目的】探讨精子DNA碎片率与顶体酶活性检测对男性不育的诊断价值。【方法】回顾性分析近三年前来本院就诊的902例男性患者的精液常规参数及精子DNA碎片率和顶体酶活性水平。【结果】按DNA碎片率水平将所有患者分为4组(≤10%,11~20%,21~30%,≥31%),各组患者的年龄、精子浓度、精子总活力、前向运动率以及顶体酶活性存在统计学差异;按精子顶体酶活性正常与否将患者分为两组后,两组间精子浓度、精子总数、精子总活力、前向运动率、正常形态率、畸形精子指数、DNA碎片率均有统计学差异。相关性分析显示,DNA碎片率与精子浓度、精子活力、前向运动率、精子总数及顶体酶活性呈负相关关系,与畸形精子指数呈正相关关系;顶体酶与精子浓度、精子活力、前向运动率、精子总数、正常形态率呈正相关关系,与头部异常率、畸形精子指数、DNA碎片率负相关。【结论】精子DNA碎片率及顶体酶活性水平均可以在一定程度上反映精子参数水平,其中DFI更能反映精子活力水平,顶体酶活性则与精子形态更为相关。两者都是精子质量评估更为客观而全面的指标。
【Objective】We aimed to explore the diagnostic value of DNA fragmentation index(DFI)and acrosin activi-ty in male infertility.【Methods】Nine hundred and two semen samples were collected from patients and assessed for the DNAfragmentation index by sperm chromatin dispersion test,acrosin activity,as well as standard sperm parameters according tothe WHO criteria. Statistical analysis was performed with SPSS.【Results】Statistically significant differences were observedin age,sperm concentration,total motility,progressive rate and acrosin activity among different group of sperm DNA dam-age(≤10%,11~20%,21~30%,≥31%). Sperm acrosin activity also showed difference in sperm concentration,total num-ber,total motility,progressive rate,normal morphology rate,teratozoospermia index(TZI)and sperm DFI. The DNA frag-mentation rate and sperm concentration,sperm motility,forward motility rate,total sperm count and acrosin activity was neg-atively correlated,while it is negative correlatd with TZI. Acrosin activity and sperm concentration,sperm motility,forwardmotility rate,sperm count and normal morphology rate was positively correlated,while it is negative correlated with the ab-normal rate of head,sperm deformity index,DNA fragmentation rate.【Conclusion】Sperm DNA damage and acrosin activitycould partly reflect the quality of sperm. Moreover,sperm DFI maypredict the sperm motility part while the acrosin activitymore likely related to sperm morphology.
出处
《中山大学学报（医学版）》
CAS
CSCD
北大核心
2018年第1期93-100,共8页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金(2016A030313071)
关键词
男性不育
精子
DNA碎片率
顶体酶活性
male infertility
spermatozoa
DNA fragmentation index
acrosine activity
