摘要
以冷藏金枪鱼货架期终点筛选、纯化、鉴定所得优势腐败菌假单胞菌(Pseudomonas spp.)、不动杆菌(Acinetobacter spp.)和热死环丝菌(Brochothrix thermosphacta)作为研究对象,通过分析接种优势腐败菌的冷藏金枪鱼中各腐败微生物生长动态以及感官品质、挥发性盐基氮(total volatile basic nitrogen,TVB-N)含量、生物胺含量的变化,以生长动力学参数和腐败物质的产量因子YTVB-N/CFU为评价标准,分析评价冷藏金枪鱼优势腐败菌假单胞、不动杆菌和热死环丝菌的致腐败能力。结果表明:4℃冷藏金枪鱼中假单胞菌、不动杆菌和热死环丝菌的生长曲线拟合效果最好的方程分别为修正Gompertz模型、Baranyi-Roberts模型和Logistic模型,分析所得生长动力学参数发现,冷藏金枪鱼中假单胞菌、不动杆菌和热死环丝菌生长延滞时间和最大比生长速率分别为10.85、83.93、79.11 h和0.045 7、0.182 9、0.111 1 h^(-1);接种假单胞菌、不动杆菌和热死环丝菌的冷藏金枪鱼感官货架期分别为96、108 h和105 h,此时TVB-N含量分别为27.32、22.80 mg/100 g和24.85 mg/100 g;分析冷藏过程中金枪鱼生物胺变化情况发现,假单胞菌产生物胺能力强于不动杆菌和热死环丝菌,贮藏96 h时接种假单胞菌组的生物胺指数已达155.84,超过规定阈值100;冷藏金枪鱼致腐败能力分析结果显示,假单胞菌、不动杆菌和热死环丝菌的产量因子YTVB-N/CFU分别为7.36×10-8、1.85×10-8 mg TVB-N/CFU和5.07×10^(-8) mg TVB-N/CFU,研究表明4℃冷藏金枪鱼贮藏过程中假单胞菌致腐败能力较强,其次为热死环丝菌和不动杆菌。
Pseudomonas spp., Acinetobacter spp. and Brochothrix thermosphacta isolated from chilled raw tuna（Thunnus obesus） at the end of shelf life were explored for their spoilage potential. Bacterial counts and growth kinetic parameters, sensory quality, total volatile basic nitrogen（TVB-N） and biogenic amine contents and the yield factor of metabolic products, YTVB-N/CFU, were assayed on sterile T. obesus inoculated separately with each of the three isolates during chilled storage. It was found that the modified Gompertz, Baranyi-Roberts, and Logisitic models could describe the dynamic growth of Pseudomonasspp., Acinetobacter spp. and Brochothrix thermosphacta at cold temperature. The Lag phase of the 3 bacterial strains were 10.85, 83.93 and 79.11 h, respectively, and the relative maximum growth rates were 0.045 7, 0.182 9 and 0.111 1 h^（-1）, respectively. The shelf life of inoculated tuna was 96, 108 and 105 h, respectively. The TVB-N contents of sterile T. obesusinoculated with Pseudomonas spp., Acinetobacter spp. and B. thermosphacta were 27.32, 22.80 and 24.85 mg/100 g, respectively. The changes in biogenic amines contents of T. obesus during chilled storage revealed that the capability of Pseudomonas spp. to produce biogenic amines was stronger than that of Acinetobacter spp. and B. thermosphacta. The biogenic amine index of tuna inoculated with Pseudomonas spp. was 155.84 after 96 h storage, which was higher than the threshold value of 100. This study also showed that YTVB-N/CFU values for the 3 strains were 7.36 × 10-8, 1.85 × 10-8 and 5.07 × 10^（-8） mg TVB-N/CFU, respectively, indicating that Pseudomonas spp. played the most important role in spoilage of chilled raw T. obesus, followed by B. thermosphacta and Acinetobacter spp..
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第3期7-14,共8页
Food Science
基金
国家自然科学基金面上项目(31571914)
2016年上海市科技兴农重点攻关项目[沪农科攻字(2016)第1-1号]
上海市科委平台能力提升项目(16DZ2280300)
关键词
金枪鱼
优势腐败菌
生长曲线
生物胺
挥发性盐基氮
致腐能力
tuna
dominant spoilage bacterial
growth curve
biogenic amine
total volatile basic nitrogen
spoilage potential
