摘要
为探讨中药马齿苋与益生菌联合应用对动物腹泻的预防作用,以马齿苋、益生菌为试验对象,将144只雄性昆明种小鼠随机分为9组,包括不同剂量马齿苋(1.0、2.5、5.0 g·kg^(-1)feed)组、不同剂量马齿苋(1.0、2.5、5.0 g·kg^(-1)feed)+益生菌组(2×10~6cfu·g^(-1)BW·d^(-1))、益生菌组(2×10~6cfu·g^(-1)BW·d^(-1))、模型组和空白组。模型组和空白组小鼠喂饲普通饲料,空白组不造模;其他各组小鼠或喂饲含药饲料或/和灌胃益生菌10 d后,采用灌服干姜水提液结合腹腔注射产肠毒素大肠埃希菌(ETEC)的方法构建小鼠腹泻模型。攻菌后密切观察小鼠腹泻发生情况;试验结束时,采集小鼠血液进行生化检查,采集肠道组织进行组织病理学检查及TNF-α、IL-1β、IL-6、IL-10、TGF-β1、IFN-γ等炎症因子表达检测。结果表明:(1)模型组小鼠在攻菌后24 h的腹泻率高达87.5%,高剂量马齿苋+益生菌组小鼠的腹泻率仅为12.5%;(2)模型组肠道组织中TNF-α、IL-1β、IL-10、IL-6、TGF-β1、IFN-γmRNA表达量较空白组均显著升高(P<0.05),而高剂量马齿苋+益生菌组上述炎症因子的表达量较模型组均显著降低(P<0.05);(3)模型组小鼠小肠绒毛断裂、部分坏死脱落,肠腺变形明显,而高剂量马齿苋+益生菌组小鼠肠绒毛基本完整、肠道组织结构趋于正常;(4)模型组小鼠血清中Cl^-含量较空白组显著升高(P<0.05)、Ca^(2+)含量则显著降低(P<0.05),而高剂量马齿苋+益生菌组小鼠血清中Cl^-较模型组显著降低(P<0.05)、Ca^(2+)含量则显著升高(P<0.05);可见,高剂量马齿苋与益生菌联用能有效预防造模小鼠腹泻的发生,其机制是,通过下调肠道相关炎症因子的表达减轻肠道炎症反应,调节血液Cl^-、Ca^(2+)含量恢复其电解质平衡。
In order to investigate the effect of combined application of purslane and probiotics on prevention of heattoxin syndrome diarrhea in mice,purslane and probiotics were used in this study. 144 male Kunming mice were randomly divided into 9 groups,namely: different dose of purslane( 1,2. 5,5. 0 g·kg^(-1) feed) groups,different doses of purslane( 1,2. 5,5. 0 g·kg^(-1) feed) and probiotics( 2 × 10~6 cfu·g^(-1) BW·d^(-1)) groups,probiotic( 2 × 10~6 cfu·g^(-1) BW·d^(-1)) group,model group and blank group. The model group and blank group mice were fed normal diet and blank group mice did not build model; Other mice were fed with medicated feed or/and intragastric probiotics. After10 d treatment with drugs and/or probiotics,except blank group,the diarrhea model of the other group mice was con-structed by gavage once a day with dried ginger extract for 10 days then intraperitoneal injection enterotoxigenic Escherichia coli( ETEC) for 3 days. The occurrence of diarrhea in mice was closely observed after attack bacteria. At the end of the experiment,blood samples were collected for biochemical examination,intestinal tissues were collected for histopathological examination and detection of inflammatory factors expression such as TNF-α、IL-1β、IL-6、IL-10、TGF-β1、IFN-γ. The results were as follow:( 1) The diarrhea rate of mice in the model group was 87. 5% and the diarrhea rate in the high dose purslane combined with probiotic group was just 12. 5% at 24 h after attack bacteria.( 2) Compared with the blank group,the mRNA expression of TNF-α,IL-1β,IL-10,IL-6,TGF-β1,IFN-γ were significantly increased in model group( P < 0. 05). Compared with the model group,the mRNA expression of the inflammatory factors mentioned above were significantly decreased( P < 0. 05) in the high dose purslane combined with probiotic group.( 3) In the model group,the small intestine villi of mice were broken,partially necrotic and exfoliated,and the intestinal glands were deformed obviously. The intestinal villi of the high dose purslane combined with probiotics group were basically complete and the intestinal structure became normal.( 4) Compared with the blank group,the content of Cl^- in serum of model group increased significantly( P < 0. 05),and the content of Ca^(2+)decreased significantly( P < 0. 05). Compared with the model group,the content of Cl-in serum of the high dose purslane combined with probiotics group decreased significantly( P < 0. 05) and the content of Ca^(2+)increased significantly( P < 0. 05). The results showed that the high dose purslane combined with probiotics could effectively prevent the occurrence of diarrhea in mice induced by ETEC and dried ginger extract. Its mechanism included reducing intestinal inflammation by down-regulating the expression of gut associated inflammatory factors and restoring the electrolyte balance by regulating the blood Cl^- and Ca^(2+)levels.
出处
《浙江农业学报》
CSCD
北大核心
2018年第2期211-219,共9页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(31172358)
2013年安徽省大学生创新创业训练项目(AH201310364033)
关键词
马齿苋-益生菌联用
产肠毒素大肠埃希菌
小鼠
腹泻模型
combination of purslane and probiotics
enterotoxigenic Escherichia coli
mice
diarrhea model
