摘要
目的探讨INK4位点反义长链非编码RNA（ANRIL）在肝癌组织中的表达及其对肝癌细胞增殖、迁移能力的影响。方法选取我院2014年2月至2016年6月手术切除的28例肝癌组织、癌旁组织为研究材料，采用实时定量聚合酶链反应（Real-time PCR）检测癌组织/癌旁组织中ANRIL分子的表达。采用小干扰RNA技术（siRNA）下调人肝癌细胞HepG2中ANRIL的表达，应用实验和Transwell实验分别检测下调前后细胞的增殖能力和迁移能力有无变化。结果肝癌组织中ANRIL的相对表达量为1.82±0.31，显著高于癌旁组织中的0.89±0.34（P＝0.020）；siRNA能够成功下调HepG2细胞中ANRIL的表达；抑制HepG2细胞中ANRIL表达后，细胞的增殖能力显著降低（P＝0.010）；而侵袭能力无显著变化（P＝0.670）。结论长链非编码RNA ANRIL可能在肝癌发生与发展中发挥重要作用。
Objective To evaluate growth inhibition of hepatocellular carcinoma by RNA interference targeting antisense non-coding RNA in the INK4 locus （ANRIL）.Methods Twenty eight hepatocellular carcinoma patients who received surgery treatment were included in this study. ANRIL relative expression was tested by real-time quantitative polymerase chain reaction （Real-time PCR） assay in cancer and normal liver tissue. Expression of ANRIL was knocked down by small interference RNA in human hepatocellular carcinoma HepG2 cell line. Cell proliferation and migration ability was tested by cell counting kit-8 （CCK-8） assay and transwell assay.Results ANRIL relative expression were 1.82±0.31 and 0.89±0.34 for cancer and normal tissue with statistical difference （P=0.025）; small interference RNA can successfully know down ANRIL expression in HepG2 cell line; After knocking down ANRIL expression in HepG2 cell, the proliferation ability was inhibited （P=0.010）. However, the migration ability was not affected by inhibiting ANRIL expression in HepG2 cells （P=0.670）.Conclusion ANRIL may play an important role in the development of human hepatocellular carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第2期331-333,共3页
Chinese Journal of Experimental Surgery
关键词
INK4位点反义长链非编码RNA
肝细胞癌
增殖
迁移
Antisense non -coding RNA in the INK4 locus
Hepatocellular carcinoma
Proliferation
Migration
