摘要
目的:分析128例不规则抗体筛查阳性患者的Rh抗原分型以及不规则抗体鉴定结果,探讨需要多次输血患者首次输血前进行Rh抗原分型检测的临床意义。方法:采用试管法对128例抗筛阳性患者的Rh抗原进行分型检测;采用微柱凝胶法对该类患者进行单特异性抗体鉴定。结果:128例抗体筛查阳性的患者经抗体鉴定,Rh系统共77例,其中抗E有72例,抗c有5例;MNSs系统抗M有10例,抗Mur有4例;Lewis系统抗Lea有15例;P系统抗P1有4例,其他非特异性抗体18例。Rh抗原分型检测分布为DCCee(74例)>DCc Ee(34例)>DCcee(10例)>Dcc EE(5例)>Dcc Ee(2例)>DCc EE(1例)、d Ccee(1例)、dccee(1例),产生Rh系统抗体的表型主要是DCCee,其患者主要分布在血液内科(26例)、消化内科(11例)、ICU(4例)等需要反复输血的病区。结论:针对血液内科、消化内科、ICU等病区需要反复输血的患者,在首次输血前进行Rh抗原分型检测,尽量选用Rh系统五个抗原同型的血液给予输注,可以避免患者产生该系统的不规则抗体,从而保证安全输血和有效输血。
Objective： The Rh antigen typing and antibody identification results of the patients whose irregular antibody screening positive were analyzed,to explore the clinical significance of detecting the Rh antigens typing before the first blood transfusion of the patients who require multiple blood transfusions. Methods： The Rh antigenic typing of 128 irregular antibody screening positive patients were tested by test tube method. The monospecific antibody were identified by microcolumn gel method. Results： Of the 128 patients with irregular antibody screening positive,there were 77 cases in Rh system,including 72 cases of anti-E and 5 cases of anti-c. There were 14 cases of MNSs system,including 10 cases of anti-M and 4 cases of anti-Mur. There were 15 cases of anti-Leain Lewis system. There were 4 cases of anti-P1 in P system and 18 cases of other nonspecific antibodies. The distribution of Rh antigen detection was DCCee（ 74 cases） DCc Ee（ 34 cases） DCcee（ 10 cases） Dcc EE（ 5 cases） Dcc Ee（ 2 cases） DCc EE（ 1 case）,d Ccee（ 1 case）,dccee（ 1 case）. The majority phenotype of Rh system antibodies in patients were DCCee. The patients were mainly distributed in the wards who require repeated blood transfusions such as the department of blood internal medicine（ 26 cases）,digestive internal medicine（ 11 cases）,ICU（ 4 cases）. Conclusion： Before the first blood transfusion,we detect the Rh antigenic typing and choose the same antigen phenotype of Rh system for the patients who require blood transfusions repeatedly,which can avoid producing the irregular antibodies in this system,and then to ensure the safety and effective of the blood transfusion.
作者
阳志勇
陈芝喜
YANG Zhi-Yong;CHEN Zhi-Xi(Department of Blood Transfusion,the First Affiliated Hospital of University of SouthChina,Hengyang 421001,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2018年第2期267-269,共3页
Chinese Journal of Immunology
关键词
不规则抗体
Rh抗原分型
抗体筛查
抗体鉴定
反复输血
Irregular antibody
Rh antigenic typing
Antibody screening
Antibody identification
Blood transfusions repeatedly
