摘要
潘糖是低聚异麦芽糖中的关键组分,具有较高的科研利用价值.但是其价格昂贵,且在市场上不易购买,限制了低聚异麦芽糖的相关研究与应用.本研究通过高效液相色谱-蒸发光散射检测法对低聚异麦芽糖产品的全组分进行定性分析,鉴定了其主要功能性成分.采用高效液相色谱-示差折光检测法对关键组分潘糖进行分离纯化.所获得潘糖的平均纯度、平均回收率、精密度实验的相对标准偏差和重复性实验的相对标准偏差分别为(98.43±0.25)%、(96.20±0.73)%、0.25%和0.27%,,并且它可在2~8℃条件下稳定保存至少12个月.因此,该潘糖产品符合2015年版中华人民共和国药典的相关规定,可作为二级标准品使用.这为低聚异麦芽糖的相关研究与应用提供了基础材料.
Panose, the key component of isomaltooligosaccharides（IMO）, is of great scientific and practical interest in many fields. However, it is very expensive and can not be easily bought in the market, which restricts the developmentand application of IMO. In this study, high performance liquid chromatography-evaporative light scattering detection（HPLC-ELSD）was adopted to qualitatively analyze all components in IMO samplesand identify the functional ingredients involved. Subsequently, high performance liquid chromatography-refractive index detection（HPLC-RID）was used to sepa-rate and purify panose from IMO samples. The purity, average recovery ratio, relative standard deviations（RSDs）for repeti-tive and precision experiments of the panose obtained were determined to be（98.43±0.25）%,, （96.20±0.73）%,, 0.25%, and 0.27%,, respectively. Besides, the purified panose could be kept at 2-8,℃ for more than 12,months. Therefore, the panose prepared in this study complied with Pharmacopoeia of People’s Republic of China（2015,Edition）and could be used as sec-ondary standard. Knowledge gained here provides a fundamental basis for the in-depth study of IMO.
作者
李玮
董自星
李普均
金鹏
刘晓光
路福平
王正祥
LI Wei;DONG Zixing;LI Pujun;JIN Peng;LIU Xiaoguang;LU Fuping;WANG Zhengxiang(Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science ＆ Technology, Tianjin 300457, China;College of Chemical Engineering and Materials Science, Tianjin University of Science ＆ Technology, Tianjin 300457, China)
出处
《天津科技大学学报》
CAS
2018年第1期25-29,共5页
Journal of Tianjin University of Science & Technology
关键词
低聚异麦芽糖
潘糖
分离纯化
标准品
制备
isomaltooligosaccharides
panose
separation and purification
standard
preparation
