摘要
目的探讨虾青素对血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导的心脏成纤维细胞(cardiac fibroblasts,CFs)胶原蛋白合成的影响。方法体外培养SD乳鼠的CFs,分为5组,分别为对照组、AngⅡ组、AngⅡ+虾青素低剂量组(20μmol/L)、AngⅡ+虾青素中剂量组(40μmol/L)、AngⅡ组+虾青素高剂量组(80μmol/L),干预24h后,采用Western blot法检测Ⅰ型胶原蛋白的含量,RT-PCR检测α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的含量,活细胞计数试剂盒(CCK8)测定CFs增殖情况。结果 AngⅡ可以使Ⅰ型胶原蛋白和α-平滑肌肌动蛋白表达上升(P<0.05),CFs增殖增加,不同浓度的虾青素均可以抑制这种作用(P<0.05)。结论在一定的浓度范围内,虾青素可抑制心脏成纤维细胞胶原蛋白的合成,抑制心脏成纤维细胞的增殖。
Objective To investigate the effect of astaxanthin on the collagen expression in cardiac fihroblasts induced by Angloten- sin 1I （Ang I/ ）. Methods We cultured cardiac fibroblasts（CFs） of neonatal Sprague- Dawley（SD） rats in vitro. The CFs were divided into five groups which were control group cultured without Ang Ⅱ or astaxanthin, Ang I1 group cultured with Ang Ⅱ and astaxanthin groups cultured with Ang Ⅱ and astaxanthin 20,40,801xmol/L, respectively. After 24 hours of cultivation, the level of collagen Ⅰ was detected by Western bloting, the expression of the α -smooth muscle aetin （ct -SMA）was detected by reverse transcription polymerase chain reaction （ RT - PCR） , and the proliferation of CFs was measured by CCK8 kit. Results The expression of collagen I and a - SMA were increased induced by Ang I1 （ P 〈 0.05 ） , and the proliferation of CFs was also enhanced by Ang 11 , but astaxanthin reversed these effects during co - treating with Ang Ⅱ （P 〈 0.05 ）. Conclusion Within a range of given concentrations, astaxanthin can reduce the expression of collagen I and Ⅱ -SMA, and inhibit the proliferation of CFs, which indicate that astaxanthin can inhibit cardiac fibrosis.
出处
《医学研究杂志》
2018年第2期36-40,共5页
Journal of Medical Research
基金
国家自然科学基金资助项目(81170085)
