摘要
目的研究回阳生肌拆方(益气温阳拆方和活血通络拆方)对糖尿病小鼠慢性皮肤溃疡创面愈合及创面巨噬细胞表型转化的影响。方法 db/m小鼠10只为正常组,db/db小鼠30只,随机分为模型组、益气温阳组、活血通络组,每组10只。制备背部创面,外用药物贴敷,隔天换药,创面拍照并测定创面面积;创伤后7天处死小鼠背部取创面组织。采用苏木素-伊红(HE)和马松(Masson)染色法对创面组织染色并观察病理学改变;采用实时定量PCR法检测创面局部M1型与M2型细胞因子mRNA相对表达。结果模型组创面面积明显大于正常组(P<0.05);回阳生肌益气温阳拆方与活血通络拆方组创面面积均明显小于模型组(P<0.05)。模型组IL-12、TNF-αmRNA表达水平高于正常组(P<0.05),VEGF、TGF-βmRNA表达水平低于正常组(P<0.05);益气温阳与活血通络组IL-12、TNF-αmRNA表达水平低于模型组(P<0.05),VEGF、TGF-βmRNA表达水平高于模型组(P<0.05)。益气温阳组IL-12、TNF-αmRNA表达水平低于活血通络组(P<0.05),TGF-βmRNA表达水平高于活血通络组(P<0.05)。结论 db/db小鼠存在创面愈合迟缓;回阳生肌拆方可促进db/db小鼠创面愈合;减少M1型巨噬细胞含量,升高M2型巨噬细胞含量,可能是其作用机制之一;对于糖尿病慢性皮肤溃疡小鼠,益气温阳拆方促进巨噬细胞表型转化作用优于活血通络拆方。
Objective To study the effect of modified formulae for Huiyangshengji ointment （YQWY and HXTL） on wound healing and macrophage phenotype transformation in diabetic mice. Methods 10db/m mice regarded as normal group, 40 db/db mice were ran- domly divided into the model group, YQWY group and HXTL group, 10 in each group. Surgical operation on the back was made to pre- pare experimental wound models. External medicines were applied and changed dressings every other day, with wound photographs and measurement. Wound tissues were gained when animals were sacrificed by anesthesia 7 days later. Pathological changes were observed by HE and Masson staining methods, while RT - PCR method was used to detect the mRNA expressions of M1/M2 type cytokines of the wound tissues. Results Blood sugar of db/db was higher than the db/m （ the normal group）. Compared with the normal group, the wound area of db/db was significantly greater than the normal group （ P 〈 0.05 ）, while YQWY group and HXTL group less than the mod- el group （P 〈 0.05 ）. Relative expression of IL - 12mRNA and TNF - α mRNA in model group were higher than the normal group （P 〈 0.05） , while VEGFmRNA and TGF -βmRNA lower. Compared with the model group, mRNA relative expression of inflammatory cyto- kines of YQWY and HXTL groups were all lower（ P 〈 0.05 ） , while the growth factor were significantly higher （ P 〈 0.05 ）. Expressions of IL - 12mRNA and TNF - α mRNA in YQWY group were lower than the HXTL group （P 〈 0.05 ） , with higher expression of TGF - βmRNA （P 〈 0.05）. Conclusion Wound healing of db/db mice was delayed and modified formulae for Huiyangshengji ointment （YQWY and HXTL） had effects on wound healing. The way by decreasing the M1 type inflammatory factors and improving the M2 type growth factors might be one of the mechanisms for wound healing. YQWY group was superior than HXTL group for diabetic mice in promo- ting the transformation of macrophage phenotype.
出处
《医学研究杂志》
2018年第2期53-57,68,共6页
Journal of Medical Research
基金
国家自然科学基金资助项目(81273768
81403409
81403270)
