摘要
目的：探讨纳米氢氧化钙根管充填材料的制备及其生物功能性和安全性。方法：改良制备纳米氢氧化钙的装置,以CaCl_2和NaOH为沉淀反应物,聚乙二醇为改性剂,超声波为辅助振动,正丁醇共沸蒸馏法干燥分散制得纳米氢氧化钙粉体,将粉体与医用碘仿和甘油调制成适合临床根管治疗要求根充糊剂,对制备粉体进行表征和葡萄糖微渗漏检测,以及与人体红细胞反应的溶血状况。结果：本实验搭建的制备装置自动化程度高,隔菌防尘,明显缩短制备时间,节省了人力和物力;本方法能够制备出具有明显氢氧化钙和聚乙二醇特征吸收波峰,粒径在100-200nm,大小均匀分散,符合临床根管治疗要求的纳米氢氧化钙;充填糊剂能够显著防止微渗漏发生,在使用初期几乎没有微渗漏发生,随时间延长,微渗漏值增加并不明显,与临床上常规使用的氢氧化钙根充糊剂Vitapex比较,在相同时间段,其防止葡萄糖微渗漏值显著减小,在第28天,葡萄糖微渗漏值为（2.09±0.89）mmol/L显著低于Vitapex的（5.04±0.98）mmol/L（P〈0.01）。纳米氢氧化钙根管充填材料使红细胞结构完整,不引起红细胞破裂而溶血,其造成血红蛋白含量的相对值明显低于阳性对照组（P〈0.01）,溶血率为4.41%,显著低于医用置入材料引起红细胞溶血率允许范围。结论：本实验改良的制备装置科学合理,制备的粉体为纳米级、分散均匀的氢氧化钙,作为根充材料具有显著防止微渗漏作用和不引起溶血的生物安全性,是一种值得开发的新型、具有发展前途的纳米充填材料。
Objective： To investigate the upgrade of manufacturing systems of nanometer calcium hy- droxide （Ca（OH）2） root canal filling material and their effects on biological functionality and bio- logical safety. Methods： A nano-Ca（OH）2 by use of synthetic nano-precipitation titration of Ca （OH）2 materials with the raw materials for CaC12 and NaOH as well as polyethylene glycol （PEG） was produced. At whole procedure of producing nano-Ca（OH）2, PEG firstly parceled CaC12 and reacted with NaOH, ultrasonic wave was used to vibrate reaction so that to accelerate production of nano-Ca（OH）2 and prevent agglomeration of particles, as well as drying method of heterogeneous azeotropic distillation was made to divergence the nano-Ca（OH）2 gel. Nano-Ca （（）H）2 root canal filling material came from mixing with medical iodoform and glycerol with same the requirements of clinical root canal therapy. The infrared spectroscopy and transmission elec- tron microscopy were used to detect the physical and chemical characteristics of nano-Ca（OH）2, and sealing root canal system of nano-Ca（OH）2 root canal filling material was evaluate by the use of endodontic microleakage model with glucose-enzymatic oxides. In addition, hemolysis proper- ties were tested by contacting with human red blood cells. Results： The results were illustrated by the improved method of producing nano-Ca（OH）2 with high automaticity isolation from bacte- rial microorganisms and dust particles, as well as decreased manufacture time, work intensity and cost. The wave peak with Ca（OH）2 and PEG in material elements was detected and was at last sured to be Ca（OH）2 material, and nano-particle of Ca（OH）2 was only 100-200 nanometer. The current new nano-Ca（OH）2 root canal filling material had compact structure and smooth surface, which was better than ordinary Ca（OH）z root canal filling material. The nano-Ca（OH）2 root ca- nal filling material could significantly prevent the endodontic microleakage so that it had an effects same as the sealed root canal system, and also had no obvious increase of microleakage value with time prolongation. The value of endodontic microleakage was markedly decreased by using nano- Ca（OH）2 root canal filling material with（2.09±0.89）mmol/L compared with ordinary Ca（OH）2 root canal filling material like Vitapex with（5.04±0.98）mmol/L on Day 28（P〈0.01）. Nano-Ca （OH）2 root canal filling material did not interact with hemoglobin and then maintained the mor- phological integrity and smooth surface of red blood cells. Hemoglobin value was significantly de- creased as compared with control group （P〈0.01）, and with 4.41% of the complete hemolysis rate, which was lower than that of conventional medical root canal filling material. Conclusion： Upgraded manufacture method of nano-Ca（OH）2 might be scientific and reasonable, and could produce nano-level, evenly dispersed uCa（OH）2, as well as prevent endodontic microleakage and hemolysis of human red blood cells. It might contribute to develop the novel and outlook nano- root canal filling material for clinic root canal therapy in future.
出处
《武汉大学学报（医学版）》
CAS
2018年第2期273-278,共6页
Medical Journal of Wuhan University
基金
国家大学生创新创业基金资助项目(编号:201610486111)
关键词
纳米氢氧化钙
根管充填材料
制备方法
表征
根管微渗漏
生物相容性
Nanometer Calcium Hydroxide
Root Canal Filling Material
Manufacturing Method
Physical Characterization~ Endodontic Microleakage
Biocompatibility
