摘要
目的:探寻油剂体外杀菌效果的检测方法,同时检测臭氧化山茶油对金黄色葡萄球菌的体外杀伤效果。方法:制备金黄色葡萄球菌悬液,采用三区划线法将金黄色葡萄球菌悬液接种于LB平板,培养过夜后选取21个直径相同的细菌单克隆,平均分为3组:对照组、基础油(山茶油)组、臭氧油(臭氧化山茶油)组。用无菌环隔离细菌单克隆,并给予臭氧油等处理,将平板正置培养过夜,次日挑取隔离培养的整个细菌单克隆于5 m L液体LB培基中培养12 h,然后采取平板计数法和比浊法检测菌液浓度,从而判断臭氧油的杀菌效果。结果:平板计数法和浊度法均显示,经臭氧油处理后的单克隆重悬培养后,菌液的细菌浓度显著低于对照组和基础油组(P<0.001)。结论:臭氧化山茶油对金黄色葡萄球菌有显著杀伤作用,单克隆菌落培养法检测油剂体外杀菌作用方法学可靠。
Objective： To explore a new method for detecting the bactericidal effect of oiling agent in vitro, and to determine the disinfectant effecacy ofozonated camellia oil on Staphylococcus aureus. Methods： Suspension of Staphylococcus aureus was prepared and innoculated on the LB plate by plate scribing method. After culture overnight, 21 bacterial monoclones with the same diameter were selected and divided into 3 groups： A negative control group, a baseoil （camellia oil） group and an ozonated camellia oil group. We used a ring to isolate the single clone and added oil inside the ring, cultured the whole plate over night, picked out each single clone （with gel） to 5 mL LB medium and cultured it for 12 h. The final concentration of the LB medium was detected by plate count method and turbidimetry.Results： According to the plate count method and turbidimetry3 the bacterial concentration in the ozonated camellia oil group was lower than that （P〈0.001）. in the negative control group and base oil group Conclusion： Bacterial monoclone culture method shows that ozonated camellia oil can significantly kill Staphylococcus aureus, and this method is an effective method for evaluating the bactericidal function of the oiling agent in vitro.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2018年第2期139-142,共4页
Journal of Central South University ：Medical Science
基金
湖南省自然科学基金(2015JJ6120)
湖南省卫生计生委科研计划项目(B2015-34)
湖南省中医药管理局项目(201520)~~
