摘要
目的探讨乳腺癌患者外周血miRNA-134表达及化疗耐药的机制。方法将98例初诊乳腺癌患者根据化疗情况分为化疗敏感组(68例)和化疗耐药组(30例),采用RT-PCR检测外周血miRNA-134表达。将miRNA-mimics、miRNA-inhibitor转染至表柔比星和多西他赛耐药细胞株,检测细胞凋亡情况及Bax、Caspase-3蛋白表达水平。结果化疗敏感组血清miRNA-134表达水平明显高于化疗耐药组(P<0.05);表柔比星耐药和多西他赛耐药细胞增殖能力明显低于MCF-7细胞(P<0.05)。与正常MCF-7细胞系比较,表柔比星耐药细胞系、多西他赛耐药细胞系miRNA-134表达水平均明显降低(P<0.05)。转染miRNA-mimics细胞凋亡率明显高于未转染细胞和细胞miRNA-inhibitor细胞(P<0.05)。转染miRNA-mimics细胞Bax、Caspase-3蛋白表达水平明显高于未转染细胞和转染miRNA-inhibitor细胞(P<0.05)。结论 miRNA-134可能通过促进Bax、Caspase-3的表达,增强乳腺癌细胞对化疗的敏感性。
Objective To explore the expression of peripheral blood miRNA-134 in the patients with breast cancer and chem- otherapy drug resistance mechanism. Methods A total of 98 cases of newly diagnosed breast cancer were divided into the chemo- therapy sensitive group（n= 58） and chemotherapy drug resistant group（n=30）. The expression of miRNA-134 in peripheral blood was measured by adopting RT-PCR. miRNA-mimics and miRNA-inhibitor were transfeeted into epirubiein and docetaxel resistant cell lines, the cell apoptosis and expression levels of Bax and Caspase-3 protein were detected. Results The ievel of serum!haiRNA- 134 expression in the chemotherapy sensitive group was significantly higher than that in the chemotherapy drug resistant group （P〈0.05） ;the proliferation capacity of epirubicin and docetaxel resistant cell lines was significantly lower than that in the MCF-7 cells （P〈0, 05）. Compared with normal MCF-7 cell line, the expression level of miRNA-134 in the epirubicin and docetaxel resistant cell lines was significantly decreased（P〈0.05）. The apoptosis rate in the miRNA-mimics group was significantly higher than that in the control group and miRNA-inhibitor group（P〈0.05）. The expression of Bax and Caspase-3 protein in the miRNA-mimics group was significantly higher than that in the control group and miRNA-inhibitor group（P〈0.05）. Conclusion MiRNA-134 may enhance the sensitivity of breast cancer cells to chemotherapy by promoting the expression of Bax and Caspase-3.
出处
《重庆医学》
CAS
2018年第6期779-782,785,共5页
Chongqing medicine
