摘要
【目的】探讨ARHGAP11A基因与南江黄羊生长性状的关联。【方法】以南江黄羊(母羊,n=243)为试验材料,采用PCR产物直接测序筛选ARHGAP11A基因的SNP位点并分析其与性状之间的关联。【结果】在南江黄羊群体中共发现ARHGAP11A基因16个SNPs位点。g.3483A>G和g.3621G>A的纯合突变具有增加南江黄羊体重(2月龄、6月龄和12月龄)以及胸围(6月龄)指标的趋势(P>0.05),而在g.3525A>G位点AG型羊初生重、12月龄体重和12月龄胸围显著高于GG型个体(P<0.05)。AAGT单倍型山羊体重体尺均高于AGGT和GGAT个体(P>0.05)。【结论】南江黄羊母羊群体中ARHGAP11A基因突变与生长发育密切相关,进一步验证其功能后在选育中可考虑其作为辅助选择标记。
【Objective】The objective of this study was to investigate the linkage between ARHGAP11 A gene and the growth traits of livestock.【Method】Using Nanjiang Yellow goats(female,n=243) as experimental materials,we then detected the single nucleotide polymorphisms(SNPs) in ARHGAP11 A gene by direct sequencing and analyzed the potential effects of these SNPs with the growth traits.【Results】A total of 16 novel SNPs of ARHGAP11 A gene were identified in Nanjiang Yellow goats. In addition,homozygous mutations at g.3483 A >G and g.3621 G >A were related with increased body weight(2 months,6 months and 12 months) and chest circumference(6 months)(P>0.05). Meanwhile at g.3525 A>G,individuals with AG genotype were significantly larger than those of GG-genotype goats,especially body weight at birth and 12 month and chest circumference at 12 month(P<0.05). Furthermore,compared with AGGT and GGAT goats,individuals with AAGT-haplotype tended to be dominant at body mass and size( P >0.05). 【Conclusion】 These results preliminarily elucidate that polymorphisms of ARHGAP11 A gene are closely related to the development of female Nanjiang Yellow goats,which could be used as molecular marker in breeding when the effects of these mutations are further proved.
出处
《四川农业大学学报》
CSCD
北大核心
2018年第1期91-99,共9页
Journal of Sichuan Agricultural University
基金
四川省科技支撑计划(2016NYZ0045)
西藏自治区科技重大专项(Z2016B01N04-06)
