摘要
目的探讨活血潜阳祛痰方对肥胖高血压大鼠血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)含量、稳态模型胰岛素抵抗指数(homeostatic model assessment for insulin resistance,HOME-IR)及血脂的干预作用。方法 5周龄雄性自发性高血压大鼠(spontaneous hypertensive rat,SHR)高脂饮食10周以复制肥胖高血压模型,再将肥胖高血压大鼠随机分为模型组、中药组和西药组,另设置京都大鼠对照组和SHR对照组。中药组、西药组、其余3组分别以活血潜阳祛痰方、缬沙坦、正常饮用水灌胃。治疗8周后观察体质量、收缩压(systolic blood pressure,SBP),测定血清和心肌AngⅡ含量以及空腹血糖(fasting plasma glucose,FPG)、胰岛素和血脂水平。结果活血潜阳祛痰方可显著降低肥胖高血压大鼠升高的Lee指数、SBP、FPG、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)和HOME-IR水平(P<0.05),显著降低升高的血清和心肌AngⅡ水平(P<0.05)。结论肥胖高血压大鼠有严重的内分泌代谢异常,活血潜阳祛痰方可有效降低AngⅡ含量,改善其血脂异常及胰岛素抵抗状态。
Objective To explore the interventional effect of Huoxue Qianyang Qutan Prescription on angiotensin Ⅱ(AngⅡ),homeostatic model assessment for insulin resistance(HOME-IR),and blood lipids in rats with obesity and hypertension.Methods Five-week-old male spontaneously hypertensive rats(SHRs)were fed with high-fat diet for 10 weeks to establish a rat model of obesity and hypertension,and then these rats were randomly divided into model group,traditional Chinese medicine(TCM)group,and Western medicine group.Wistar-Kyoto rat control group and SHR control group were also established.The rats in the TCM group were given Huoxue Qianyang Qutan Prescription by gavage,those in the Western medicine group were given valsartan by gavage,and those in the other three groups were given purified drinking water by gavage.Body mass index and systolic blood pressure(SBP)were measured after8 weeks of treatment,as well as the level of AngⅡin serum and the myocardium,fasting plasma glucose(FPG),and levels of insulin and blood lipids.Results In the rats with obesity and hypertension,Huoxue Qianyang Qutan Prescription significantly reduced the increased Lee index,SBP,FPG,total cholesterol,low-density lipoprotein cholesterol,HOME-IR,and Ang Ⅱlevel in serum and the myocardium(all P<0.05).Conclusion Rats with obesity and hypertension have severe endocrine-metabolic disorder,and Huoxue Qianyang Qutan Prescription can effectively reduce the level of Ang Ⅱ and improve abnormal blood lipid levels and insulin resistance.
出处
《安徽中医药大学学报》
2018年第1期72-76,共5页
Journal of Anhui University of Chinese Medicine
基金
上海市进一步加快中医药事业发展三年(2014-2016年)行动计划项目(ZY3-CCCX-3-3026)
高等学校博士学科点专项科研基金(20133107110011)
上海市岳阳中西医结合医院高血压重点专病专科
关键词
自发性高血压大鼠
肥胖
活血潜阳祛痰方
血管紧张素Ⅱ
胰岛素抵抗
Spontaneous hypertensive rat
Obesity
Huoxue Qianyang Qutan Prescription
Angiotensin Ⅱ
Insulin resistance
