摘要
建立一种基于PCR技术的食品中猪源性成分快速检测技术。根据猪线粒体cytb基因设计引物,进行PCR扩增。该方法对猪DNA检测的灵敏度达到100pg;该方法仅对猪DNA检测呈阳性,与其他物种DNA无交叉反应;同时可用于商业样本及复杂食物样本的检测。本研究的猪源性成分特异PCR检测技术具有快速、准确的特点,且具有较高的特异性和敏感性,可作为肉制品中猪源性成分快速检测的手段。
To develop a fast identification technology based on PCR for the detection of pig ingredient in meat products,primers were designed according to the mitochondrial cytochrome-b sequences of pig, then amplified by PCR . The limit of detection was up to 100pg of pig DNA; the test was only specific for pig, and no cross-reactions were observed in other non-target species. Meanwhile, it can be used for commercial and complex food samples. In this study, the pig ingredient specific PCR detection technology has the characteristic of rapidly and accurately, and with higher specificity and sensitivity, it is a rapid approach to detect pig ingredient in meat products.
基金
大学生科技创新科研项目(国家级)
项目名称为:食品中动物源性成分快速检测技术研究
项目编号:201711439015项目名称是:吉林农业科技学院微生物学重点学科培育项目(吉农院合字2015年第012号)
