摘要
目的研究天名精Carpesium abrotanoides的萜类化学成分及其细胞毒活性。方法运用正相硅胶柱色谱、制备薄层色谱及制备型HPLC等方法进行分离纯化,并经NMR等现代波谱技术进行化合物的结构鉴定。采用MTT法研究化合物的肿瘤细胞增殖抑制活性。结果从天名精中分离鉴定了11个萜类化合物,包括6个桉烷型倍半萜:大叶土木香内酯(1)、特勒内酯(2)、异土木香内酯(3)、3α,5α-二羟基异土木香内酯(4)、异特勒内酯(5)、糙叶依瓦菊内酯(6);1个吉玛烷型倍半萜:11(13)-去氢腋生依瓦菊素(7);1个卡拉布烷型倍半萜:天名精内酯酮(8);1个少见的开环倍半萜:泊氏孔菌裂环愈创木内酯(9)和2个单萜:7,8-环氧-9-(异丁酰氧基)麝香草酚异丁酸酯(10)、10-羟基-8,9-二氧异亚丙基麝香草酚(11)。结论化合物9和11为首次从天名精属植物中分离得到,化合物3、4、6、10为首次从天名精中分离得到,首次报道了化合物4和6的13C-NMR数据。细胞毒活性研究显示,化合物1、2对人胃癌细胞HGC-27和人乳腺癌细胞MDA-MB-231的增殖有较强抑制活性,其IC50值分别为18.18、16.17μmol/L和20.65、17.62μmol/L。化合物8对人胃癌细胞HGC-27的增殖有较强抑制活性,其IC50值为24.96μmol/L。
Objective To study the terpenes from Carpesium abrotanoides and their cytotoxic activities. Methods The separations and purifications were taken by column chromatography over normal phase silica gel, preparative thin-layer chromatography and preparative HPLC. Their chemical structures were elucidated through spectroscopic analyses（NMR）. The in vitro cytotoxic activities of the isolated compounds were studied by MTT method. Results Six eudesmane-type sesquiterpenes, one germacrane-type sesquiterpene, one carabrane-type sesquiterpene and one rareseco-sesquiterpenewere obtained and determined as granilin（1）, telekin（2）, isoalantolactone（3）, 3α,5α-dihydroxyisoalantolactone（4）, isotelekin（5）, asperilin（6）, 11（13）-dehydroivaxillin（7）, carabrone（8）, and Postia secoguaianolide（9）, along with two monoterpenes 7,8-epoxy-9-（isobutyryloxy）thymolisobutyrate（10） and 10-hydroxy-8,9-dioxyisopropylidenethymol（11）. Conclusion Compounds 9 and 10 are isolated from the genus Carpesium for the first time, compounds 3, 4, 6, and 10 are separated from C. abrotanoides for the first time. And it is the first time to report the 13 C-NMR of compounds 4 and 6. Compound 1 shows cytotoxicity against HGC-27 cell and MDA-MB-231 cell with IC50 values of 18.18 μmol/L and 16.17 μmol/L. Compound 2 shows cytotoxicity against HGC-27 cell and MDA-MB-231 cell with IC50 values of 20.65 μmol/L and 17.62 μmol/L. Compound 8 shows cytotoxicity against HGC-27 cell with an IC50 value of 24.96 μmol/L.
出处
《中草药》
CAS
CSCD
北大核心
2018年第3期530-535,共6页
Chinese Traditional and Herbal Drugs
基金
湖北省自然科学基金资助项目(2014CFB692)
三峡大学人才科研启动基金项目(KJ2014B026)
