摘要
目的观察复方桂枝茯苓丸的急性与长期毒性。方法急毒实验:SD大鼠40只,分为空白对照组(生理盐水)、高、中、低剂量组(20 g/kg、40 g/kg、80 g/kg药物),连续灌胃2周,观察大鼠的急性毒性。长毒实验:SD大鼠80只,分为空白对照组(生理盐水)、高、中、低剂量组(4.4 g/kg、8.8 g/kg、17.6 g/kg药物),连续灌胃3个月,停药后恢复性观察2周,检测血液生化学、血常规及主要脏器病理学变化。结果急毒结果表明:80 g/kg下仍未观察到毒性症状。长毒结果表明,大鼠血液生化学指标(AST、TG、UREA、Glu、ALT、TC、Cr及ALP),血常规学指标(PLT、MCV、RBC、HCT、WBC、HGB、MCH、Ne及MCHC),主要脏器指标(脑、肝、心、肺、脾、肾、子宫与睾丸)均与空白对照组一致(P>0.05)。结论复方桂枝茯苓丸在临床拟用量范围内无明显毒性。
Objective To investigate the acute and long-term toxicity of fufangguizhifulinwan. Methods In acute toxicological test, the 40 SD mice are divided into blank group, high, middle and low groups, received water, 20 g/kg, 40 g/kg and 80 g/kg fufangguizhifulinwan, continue 2 weeks. After treatment, clinical symptoms and life-or-death situation are observed. In long-term toxicological test, the 80 SD mice are divided into blank group, high, middle and low groups, received water, 4.4 g/kg, 8.8 g/kg and 17.6 g/kg fufangguizhifulinwan, continue 3 months. After stopped the fufangguizhifulinwan, 2 weeks after recovery phase, blood biochemical indexes, blood routine index and main viscera histopathological are observed. Results In acute toxicity test, no death or clinical signs are found in the dose of 80 g/kg. In long-term toxicity test study, the blood biochemical indexes（AST, TG, UREA, Glu, ALT, TC, Cr and ALP）, blood routine indexes（PLT, MCV, RBC, HCT, WBC, HGB, MCH, Ne and MCHC）and main viscera histopathological（brain, liver, heart, lung, spleen, kidney, uterus and testes） in high, middle and low groups are similar with the blank group（P 0.05）. Conclusion Fufangguizhifulinwan on experimental no obvious acute toxicity in mice.
出处
《现代中药研究与实践》
CAS
2018年第1期34-38,共5页
Research and Practice on Chinese Medicines
基金
咸阳职业技术学院科研项目(2017KYB04)
关键词
复方桂枝茯苓丸
急性毒性
长期毒性
Fufangguizhifulinwan
acute toxicity test
long-term toxicity test
