摘要
目的探讨广东紫珠对脂多糖诱导巨噬细胞炎症因子分泌的影响。方法以巨噬细胞作为试验模型,以脂多糖刺激巨噬细胞模拟炎症感染状态,观察广东紫珠提取物对脂多糖诱导的巨噬细胞炎症过程的干预作用。结果与0μg/ml组相比,40μg/ml、60μg/ml、80μg/ml、100μg/ml的广东紫珠均可以显著抑制脂多糖诱导的巨噬细胞TNF-α、IL-8的分泌和NF-κB活性(均P<0.05),且存在浓度效应关系,浓度越大,抑制作用越强。结论广东紫珠治疗妇科炎症疾病的机制与其通过抑制巨噬细胞内NF-κB P65表达途径抑制脂多糖诱导的TNF-α和IL-8分泌相关。
Objective To explore the effect of Callicarpa kwangtungensis Chun on secretion of inflammation factors in macrophages induced by lipopolysaccharide. Methods Macrophages were collected as experimental model,infection status was simulated by stimulating macrophages using lipopolysaccharide,the intervention effect of Callicarpa kwangtungensis Chun extract on inflammatory process of macrophages induced by lipopolysaccharide was observed. Results Compared with 0 μg/ml group,Callicarpa kwangtungensis Chun（ 40,60,80,100 μg/ml） could significantly inhibit secretion of TNF-α and IL-8 and NF-κB activity in macrophages induced by lipopolysaccharide（ P〈0. 05）,which showed a dose-effect relationship,the higher the dose was,the stronger the inhibition effect was. Conclusion The mechanism of Callicarpa kwangtungensis Chun in treatment of gynecological inflammation is related to inhibition of secretion of TNF-α and IL-8 induced by lipopolysaccharide through inhibiting NF-κB P65 activity in macrophages.
出处
《中国妇幼保健》
CAS
2018年第3期648-650,共3页
Maternal and Child Health Care of China
基金
国家自然科学基金资助项目(81460232)
江西省卫生计生委中医药科研基金重点课题(2014Z001)
关键词
广东紫珠
巨噬细胞
炎症因子
Callicarpa kwangtungensis Chun
Macrohpage
Inflammatory factor
