摘要
目的分析白藜芦醇通过β-catenin/TCF-4信号通路对胃癌细胞增殖活性及侵袭迁移能力的影响。方法采用四甲基偶氮唑盐(MTT)实验选择适当的白藜芦醇浓度并探讨其对胃癌细胞系MGC-803生长和增殖的影响,倒置显微镜观察细胞形态学变化,采用荧光实时定量PCR(RT-PCR)法检测Wnt信号通路中β-catenin及TCF-4的m RNA表达水平,Western blot检测β-catenin、TCF-4的蛋白表达水平,Transwell检测MGC-803细胞侵袭能力的变化。结果 MTT结果显示,白藜芦醇能显著抑制MGC-803细胞的生长增殖;倒置显微镜下发现凋亡的MGC-803细胞形态发生明显变化;RT-PCR检测结果表明白藜芦醇可降低β-catenin及TCF-4的m RNA表达;Western blot检测结果表明白藜芦醇可下调β-catenin、TCF-4的蛋白表达,Transwell实验结果表明白藜芦醇使MGC-803细胞的侵袭能力显著下降。结论白藜芦醇可显著抑制胃癌细胞的增殖及侵袭能力,其作用机制可能是通过抑制β-catenin/TCF-4信号通路而实现的。
Objective To analyze the effect of resveratrol on proliferation activity and invasion ability of gastric cancer cells by regulating β-catenin/TCF-4 signaling pathway. Methods Methylthio tetrazole （MTT） assay was used to explore the appropriate concentration of res-veratrol on the growth and proliferation of gastric cancer cell line MGC-803. Morphological changes of the cells were observed by inverted microscope. The mRNA expression of β-catenin and TCF-4 in Wnt signaling pathway were detected by RT-PCR. The protein expression level of β-catenin and TCF-4 were detected by Western blot. The invasive ability changes of MGC-803 cells were detected by Transwell. Results MTT results showed that resveratrol could significantly inhibit the growth and proliferation of MGC-803 cells. The morphologi-cal changes of MGC-803 cells were observed obviously under inverted microscope. RT-PCR showed that resveratrol could decrease the mRNA expression of β-catenin and TCF-4. The western blot results showed that the protein expressions of β-catenin and TCF-4 were down-regulated by resveratrol. The Transwell results showed that resveratrol significantly decreased the invasive ability of MGC-803 cells. Conclusion Resveratrol can significantly inhibit the proliferation and invasion of gastric cancer cells, and its mechanism may be achieved by inhibiting of β-catenin/TCF-4 signaling pathway.
出处
《肿瘤药学》
CAS
2018年第1期20-25,共6页
Anti-Tumor Pharmacy
基金
新疆维吾尔自治区人民医院院内项目(20130113)
新疆维吾尔自治区自然科学基金(2014211C132)
