摘要
鉴于肝胰腺组织在凡纳滨对虾生长和免疫方面的重要生理意义,以及与对虾冷藏期间品质劣变的密切相关性,本研究通过比较不同条件下石蜡切片对凡纳滨对虾肝胰腺组织HE染色结果的影响,确定其组织化学适宜参数。凡纳滨对虾肝胰腺用Carnoy固定液在室温下固定12 h,用70%,80%,95%及无水乙醇Ⅰ各脱水30min,再继续用无水乙醇Ⅱ脱水40 min,二甲苯40 min透明2次,充分浸蜡后切片(8μm),将切片经苏木精染色40 min,盐酸乙醇分化1 s,伊红复染5 min。显微观察结果显示:细胞染色均匀,细胞核呈蓝紫色,细胞质呈紫红色,背景清晰。
Although the hepatopancreas tissue plays a vital physiological significance in growth and immunity of Litopenaeus vanname and it has a close relationship with the quality deterioration of shrimp during cold storage,the corresponding researches have been scarcely reported so far.The influence of paraffin-embedded section and staining parameters on the visualization of sections were thus investigated to seek the appropriate tissue preparation and processing.The results showed after fixation in Carnoy solution for 12 h and dehydration with the serial concentration of ethanol such as 70%,80%,95%,and 100% for 30 min respectively,the tissue was transpired twice with xylene for 40 min.With a sufficient immersing and embedding in paraffin wax,subsequent sectioning is performed on the Leica slicer with 8 μm in thickness and the obtained sections were immediately stained with hematine for 40 min.After differentiation in ethanol acidized with hydrochloric acid for 1 second,counterstaining of section with eosin for 5 min was conducted.Under these parameters the morphology of hepatopancreas sections observed with microscopy was clear in which the nuclear manifested bluish violet and sharp red was observed in cytoplasm parts.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第10期122-127,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31201309
31371746
31171634
31371777)
广东省科技厅项目(2013B021100016)
浙江省食品科学与工程重之重一级学科开放课题(JYTsp20141042)
农业部水产品加工重点实验室开放课题(NYJG201404)
广东省高等学校创新强校项目(2014KTSCX078
GDOU2013050205
GDOU2013050203)
关键词
凡纳滨对虾
肝胰腺
石蜡切片
HE染色
Litopenaeus vanname
hepatopancreas
paraffin section
hematine-eosin staining
