摘要
目的观察核连蛋白2(NUCB2/Nesfatin-1)在鼻咽癌(NPC)患者、其他头颈部肿瘤患者、鼻炎患者及健康体检者血清中的表达水平,评估NUCB2/Nesfatin-1及其联合EB病毒三项[EB病毒早期抗原抗体(EA-IgA);EB病毒核抗原抗体(VCA-IgA);Rta蛋白抗体(Rta-IgG)]诊断NPC的临床应用价值。方法收集2017年1月至2017年6月中南大学湘雅医院NPC患者、其他头颈部肿瘤患者、鼻炎患者、健康体检者血清共140例,分别采用相应试剂盒检测Nesfatin-1、EA-IgA、VCA-IgA及Rta-IgG,并进行统计学分析。结果 Nesfatin-1、EA-IgA、VCA-IgA及Rta-IgG在NPC患者血清中水平最高,与其他各组差异均具有统计学意义;Nesfatin-1与VCA-IgA及Rta-IgG呈正相关;Nesfatin-1与EA-IgA、NPC分期无相关;多元线性回归分析,回归方程为Y=208.029+17.96X_1+146.702X_2+398.879X_3(Y:Nesfatin-1;X_1:年龄;X_2:VCA-IgA;X_3:Rta-IgG;R^2=0.236);ROC曲线分析表明Nesfatin-1联合VCA-IgA及Rta-IgG诊断NPC的效率最好;单项指标评价中,Nesfatin-1的特异度最差,但敏感度最好。结论 Nesfatin-1诊断NPC敏感度极高,能弥补EA-IgA、VCA-IgA及Rta-IgG诊断NPC敏感度偏低的不足,与VCA-IgA及Rta-IgG联合应用可极大的提高诊断NPC的效能,具有较好的临床应用价值。
ObjectiveTo investigate the expression level of nucleobindin-2 （NUCB2/Nesfatin-1） in nasopharyngeal carcinoma （NPC） patients, other patients with head and neck cancer, rhinitis patients, and healthy subjects in the serum, and evaluate the clinical application value of NUCB2/Nesfatin-1 combined with three items of Epstein-Barr （EB） virus （EA-IgA; VCA-IgA; Rta-IgG） in NPC diagnosis.
MethodsFrom Xiangya Hospital of Central South University during January 2017 to June 2017, nasopharyngeal carcinoma patients, other patients with head and neck cancer, rhinitis patients, and healthy subjects samples were 140 cases, respectively. The corresponding kits were used to detect nesfatin-1, EA-IgA, VCA-IgA, and Rta-IgG.
ResultsThe serum level of nesfatin-1 in NPC patients was the highest, and the difference was statistically significant compared to other groups. The serum levels of EA-IgA, VCA-IgA, and Rta-IgG were the highest in NPC patients, and were significantly different from those in other groups. Nesfatin-1 was significantly correlated with VCA-IgA and Rta-IgG. There was no significant correlation between nesfatin-1, EA-IgA and NPC staging; Multiple linear regression analysis was used to analyze the influencing factors of nesfatin-1, and the regression equation was Y=208.029＋ 17.96X1＋ 146.702X2＋ 398.879X3 （X1： age; X2： VCA-IgA; X3： Rta-IgG; R2=0.236）. Receiver operating characteristic （ROC） curve analysis showed that nesfatin-1 combined with VCA-IgA and Rta-IgG had the best efficiency in the diagnosis of NPC. In single index evaluation, nesfatin-1 has the lowest specificity, but the highest sensitivity.
ConclusionsThe sensitivity of nesfatin-1 for NPC diagnosis is polar altitude, it can make up for the deficiency of EA-IgA, VCA-IgA, and Rta-IgG in diagnosing NPC. The combination of VCA-IgA and Rta-IgG can greatly improve the ability of diagnosing NPC.
出处
《中国医师杂志》
CAS
2018年第2期208-212,共5页
Journal of Chinese Physician
基金
2016年中南大学硕士生自主探索创新项目(2016zzts526)~~
