摘要
目的:探讨川芎不同提取物在等生药量下对异丙肾上腺素(ISO)致心肌缺血大鼠的治疗作用。方法:将正常成年大鼠分为对照组、模型组、消心痛组和川芎水提物、川芎醇提物、川芎挥发油、川芎总生物碱、川芎总酚酸组,给予预防性灌胃相应药液5d,其中各川芎提取物组给药量均相当于生药3.33g/kg,消心痛组给予消心痛9mg/kg。然后腹腔注射ISO建立大鼠急性心肌缺血模型,测定造模后1h各组大鼠心电图,试剂盒测定血清乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、天冬氨酸转氨酶(AST)、肌酸激酶(CK)活性和丙二醛(MDA)、一氧化氮(NO)、肌钙蛋白(c Tn T)的含量,蛋白免疫印迹法(western blot)测定心肌组织Bax、Bcl-2蛋白表达水平。结果:模型组大鼠心电图出现显著的J点下移和T波倒置现象,血清LDH、AST、CK活性和MDA、c Tn T含量均显著升高,SOD、NO明显下降,心肌Bax表达升高而Bcl-2表达下降,Bax/Bcl-2比例显著下降;川芎挥发油能减轻模型大鼠J点下移现象,其余各组有减缓J点下移趋势的作用,而各组均能显著改善T波倒置现象;川芎提取物能显著降低模型大鼠血清LDH、AST、MDA、CK、c Tn T活性或含量,且川芎水提物、醇提物、挥发油能纠正心肌缺血时SOD、NO的减少;各提取物组均能降低心肌Bax的表达而升高Bcl-2表达水平,从而显著降低Bax/Bcl-2比例。结论:川芎对ISO所致心肌缺血有保护作用,以川芎水提物、川芎醇提物和川芎挥发油效果最佳。
Objective： To study effects of different extracts from Ligusticum chuanxiong Hort（CX） with the same dosage of raw herb on acute myocardial ischemia（AMI） rats caused by isoprenaline（ISO）. Methods： Adult rats were divided into the control group,the model group,the positive group and different extract-groups of CX as water extract of CX（WECX） group,alcohol extract of CX（AECX）,volatile oil of CX（VOCX） group,total alkaloids of CX（TACX） group and total phenolic acids of CX（TPCX） group. Rats in extract-groups of CX were all administratered with the same dosage of 3. 33 g/kg with crude CX. Mice in the positive group were given 9 mg/kg isosorbide dinitrate. Mice in all groups were given once a day for 5 days. Then rats in all groups except the control group were intraperitoneally injected with ISO to establish AMI model. 1 h later,the ECG of all rats were determined and activities of serum lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,aspartate aminotransferase（AST）,malondialdehyde（MDA）,creatine kinase（CK）,nitric oxide（NO） and content of cardiac troponin（c Tn T） were measured with kits. The expression levels of Bax and Bcl-2 proteins in myocardium were measured by Western blot（western blot）,and changes in different groups were compared. Results： Compared with the control group,the ECG of rats in the model group in J point significantly got down and T wave inverted. Meanwhile,LDH,AST,CK activities and MDA,c Tn T in serum contents were significantly increased,SOD activity and NO level obviously decreased. The myocardial Bax expression rose and myocardial Bcl-2 expression fell. Bax/Bcl-2 had a significant reduction in the proportion Compared with the model group,VOCX reduced J point downward shift phenomenon. The rest extracts also softly raised J point. Furthermore,all extracts significantly improved T wave inversion phenomenon. All extracts significantly reduced elevated levels of LDH,AST,MDA,CK and c Tn T. Additionally,WECX,AECX,and VOCX resisted SOD and NO decrease in myocardial ischemia. all extracts also reduced the expression of Bax and increased the level of Bcl-2 in the myocardium,which finally decreased the proportion of Bax/Bcl-2. Conclusion： CX is protective for myocardial ischemia by ISO,in which WECX,AECX and VOCX are key active ingredients.
出处
《中药药理与临床》
CSCD
北大核心
2017年第5期91-94,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
四川省科技支撑计划项目(NO.15ZC0743)
四川省中医药管理局重点项目(NO.2017ZY002)
关键词
川芎
提取物
心肌缺血
Ligusticum chuanxoing （川芎）
extracts
myocardial ischemia
